EGFR Antikörper

Produktnummer ABIN372504

Der Schaf Polyklonal anti-EGFR Antikörper (ABIN372504) detektiert spezifisch EGFR in WB, IF, IHC (p), IP und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte und Huhn.

Kurzübersicht für EGFR Antikörper (ABIN372504)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reaktivität: Human, Maus, Ratte, Huhn

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser EGFR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Produktdetails anti-EGFR Antikörper

Spezifität: This antibody recognizes Human Epidermal Growth Factor Receptor.

Kreuzreaktivität (Details): Species reactivity (expected):Chicken, Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: Protein G Chromatography.

Immunogen: Recombinant 20 amino acid residues representing a portion of the internal domain of EGFR proximal to the phosphorylation region.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA. Western Blot. Immunoprecipitation. Immunocytochemistry. Immunofluorescence. Immunohistochemistry on Paraffin Sections: 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: 0.15 M PBS, pH 7.2 without preservatives

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Andere Bezeichnung: EGFR / ERBB1

Hintergrund: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The protein kinase family is one of the largest families of proteins in eukaryotes, classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.Synonyms: EGF Receptor, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, c-ErbB-1, erbB-1

Gen-ID: 1956

NCBI Accession: NP_005219

UniProt: P00533

Pathways: NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteine