XPO1 Antikörper (AA 390-408)

Produktnummer ABIN372248

Der Kaninchen Polyklonal Anti-XPO1-Antikörper wurde für WB und IHC (p) validiert. Er ist geeignet, XPO1 in Proben von Human, Maus, Ratte, Rind (Kuh), Hund und Schimpanse zu detektieren.

Kurzübersicht für XPO1 Antikörper (AA 390-408) (ABIN372248)

Target: XPO1 (Exportin 1 (XPO1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Schimpanse

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser XPO1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-XPO1 Antikörper

Bindungsspezifität: AA 390-408

Sequenz: ASPLLSGSQH FDVPPRRQL

Spezifität: This antibody recognizes CRM1 Protein (XPO1).

Kreuzreaktivität (Details): Species reactivity (expected):Bovine, Chimpanzee, Dog, Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: Protein G Chromatography.

Immunogen: Synthetic peptide corresponding to the amino acids 390-408 of human Exportin-1 protein.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on Paraffin Sections: 10 μg/mL. Western Blot: 1-3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: PBS containing 0.05 % Sodium Azide as preservative and 0.2 % Gelatin as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu XPO1

Target: XPO1 (Exportin 1 (XPO1))

Andere Bezeichnung: XPO1 / Exportin-1

Hintergrund: CRM1 (chromosome region maintenance 1 protein homolog) mediates the nuclear export of cellular proteins (cargoes) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, CRM1 binds cooperatively to the NES on its target protein and to the GTPase Ran in its active GTP-bound form. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The direction of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. CRM1 also mediates the nuclear export of the Rev protein of HIV-1 and Rex protein of HTLV-1. It is involved in Rex dimerization, U3 snoRNA transport from Cajal bodies to nucleoli. CRM1 is also involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. CRM1 is also involved in the regulation NFAT and AP-1.Synonyms: CRM1, Chromosome region maintenance 1 protein homolog, Exp1

Gen-ID: 7514

NCBI Accession: NP_003391

UniProt: O14980

Pathways: M Phase