Fibrillarin Antikörper

Produktnummer ABIN371824

Produktdetails anti-Fibrillarin Antikörper

Target: Fibrillarin (FBL)

Reaktivität: Human, Ratte, Drosophila melanogaster, C. elegans, Pflanzen, Schizosaccharomyces pombe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Fibrillarin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: Specific for the ~34 kDa Fibrillarin /Nop1p protein. This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species (e.g. 4-6).

Kreuzreaktivität (Details): Species reactivity (expected):Mammals.
Species reactivity (tested):Human, Rat, Plant, Drosophila, C. elegans and S. pombe.

Produktmerkmale: Synonyms: FIB1, FLRN, Fibrillarin, RNU3IP1, NOP1, LOT3, Nucleolar protein 1, Nucleolar Marker, rRNA2'-O-methyltransferase fibrillarin, U3 small nucleolar RNA-associated protein NOP1

Aufreinigung: Ig Fraction

Immunogen: Yeast nuclear preparations.

Klon: 38F3

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/1,000. Immunofluorescence: 1/500. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at -20 °C.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu Fibrillarin

Target: Fibrillarin (FBL)

Andere Bezeichnung: FBL (FBL Produkte)

Hintergrund: Nop1p was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5 kDa), is essential for yeast viability, and is localized in the nucleoli (1). The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archea generally called fibrillarin (2). Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67 % identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34 kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable (3).Synonyms: FIB1, FLRN, Fibrillarin, LOT3, NOP1, Nucleolar Marker, Nucleolar protein 1, RNU3IP1, U3 small nucleolar RNA-associated protein NOP1, rRNA 2'-O-methyltransferase fibrillarin

Gen-ID: 851548

UniProt: P15646

Pathways: Ribonucleoside Biosynthetic Process