PIK3C3 Antikörper (AA 700-850)

Produktnummer ABIN363642

Dieses Anti-PIK3C3-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von PIK3C3 in WB, IF und ICC. Geeignet für Human, Maus, Ratte und Schwein.

Kurzübersicht für PIK3C3 Antikörper (AA 700-850) (ABIN363642)

Target: PIK3C3 (Phosphoinositide-3-Kinase, Class 3 (PIK3C3))

Reaktivität: Human, Maus, Ratte, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PIK3C3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-PIK3C3 Antikörper

Bindungsspezifität: AA 700-850

Spezifität: Reacts with human VPS34

Kreuzreaktivität: Maus, Schwein

Kreuzreaktivität (Details): Cross reacts with proteins from rat, mouse and porcine. The immunogen sequence has 87 % homology with Xenopus protein. Not tested in other species

Aufreinigung: Antigen affinity purified

Immunogen: Synthetic peptide made to an internal portion of the human protein (within residues 700-850)

Anwendungsinformationen

Applikationshinweise:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
- WB : 1/1 000 - ICC/IF: 1/100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS, glycerol 50 % , BSA 0.1 %, Sodium azide 0.05 %

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Short term storage at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Haltbarkeit: 6 months

Details zu PIK3C3

Target: PIK3C3 (Phosphoinositide-3-Kinase, Class 3 (PIK3C3))

Andere Bezeichnung: VPS34

Hintergrund: VPS34, also known as phosphoinositide-3-kinase p100 subunit or PIK3C3 (phosphoinositide-3-kinase, class III), is a member of the PI3/PI4-kinase family. It is ubiquitously expressed with predominant expression in skeletal muscle and is believed to participate in endosome to lysosome transport, multivesicular body formation, autophagy and retrograde endosome to Golgi transport. VPS34 is the catalytic subunit of class III PI3Ks and forms a heterodimer with p150, a regulatory subunit of class 3 PI3Ks. It exclusively phosphorylates phosphatidylinositol to produce PtdIns3P. The activity of VPS34 is enhanced by manganese and can be regulated by nutrients, suggesting an important role of VPS34 in the regulation of mTOR protein synthesis. VPS34 also forms a complex with Beclin1 that promotes autophagy and tumor supression.

Pathways: AMPK Signaling, Activation of Innate immune Response, Inositol Metabolic Process, Toll-Like Receptors Cascades, Autophagie