EPAS1 Antikörper (AA 535-631)

Produktnummer ABIN363209

Der Maus Monoklonal Anti-EPAS1-Antikörper wurde für WB, IHC, ELISA, IP, ICC, FACS, IF und ChIP validiert. Er ist geeignet, EPAS1 in Proben von Human, Ratte, Maus und Hamster zu detektieren.

Kurzübersicht für EPAS1 Antikörper (AA 535-631) (ABIN363209)

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Reaktivität: Human, Ratte, Maus, Hamster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EPAS1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP)

Klon: Ep190b

Produktdetails anti-EPAS1 Antikörper

Bindungsspezifität: AA 535-631

Spezifität: Reacts with HIF-2 alpha from human, mouse hamster and rat

Kreuzreaktivität (Details): Does not cross-react with HIF-1 alpha.

Aufreinigung: Purified (protein G)

Immunogen: Human HIF-2 alpha amino acids 535-631

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
ELISA1:100-1:2000, FC1:400, IHC1:150-1:300, WB1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, Sodium azide 0.1 %

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Details zu EPAS1

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Andere Bezeichnung: Hypoxia-Inducible Factor 2-alpha

Hintergrund: Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.

Gen-ID: 2034

UniProt: Q99814

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effekt