BRCA1 Antikörper (AA 1421-1425)

Produktnummer ABIN362904

Der Kaninchen Polyklonal Anti-BRCA1-Antikörper wurde für WB und IHC validiert. Er ist geeignet, BRCA1 in Proben von Human zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für BRCA1 Antikörper (AA 1421-1425) (ABIN362904)

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BRCA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-BRCA1 Antikörper

Bindungsspezifität: AA 1421-1425

Spezifität: The antibody detects endogenous level of total BRCA1 protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.

Immunogen: Peptide sequence around AA 1421-1425 (H-G-S-Q-P) derived from Human BRCA1. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Referenzen für anti-BRCA1 Antikörper (ABIN362904)

Xiong, Li, Ai, Yin, Ouyang, Sun, Chen, Sun: "BRCA1 is required for meiotic spindle assembly and spindle assembly checkpoint activation in mouse oocytes." in: Biology of reproduction, Vol. 79, Issue 4, pp. 718-26, (2008) (PubMed).

Details zu BRCA1

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Andere Bezeichnung: BRCA1

Hintergrund: The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Plays a central role in DNA repair by facilitating cellular response to DNA repair. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation

Molekulargewicht: 220 kDa

NCBI Accession: NP_009225

UniProt: P38398

Pathways: Zellzyklus, DNA Reparatur, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus