PAK1 Antikörper (AA 210-214)

Produktnummer ABIN362773

Dieses Anti-PAK1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von PAK1 in WB, IHC und IF. Geeignet für Human, Ratte und Maus. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für PAK1 Antikörper (AA 210-214) (ABIN362773)

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PAK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-PAK1 Antikörper

Bindungsspezifität: AA 210-214

Spezifität: The antibody detects endogenous level of total PAK1protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.

Immunogen: Peptide sequence around AA 210-214 (P-V-T-P-T) derived from Human PAK1. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Referenzen für anti-PAK1 Antikörper (ABIN362773)

Wang, Ma, Xu, Wang, Zhu, An, An, Wu, Tian: "Phosphorylation of histone H3 on Ser10 by auto-phosphorylated PAK1 is not essential for chromatin condensation and meiotic progression in porcine oocytes." in: Journal of animal science and biotechnology, Vol. 4, Issue 1, pp. 13, (2013) (PubMed).

Details zu PAK1

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Andere Bezeichnung: PAK1

Hintergrund: The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.

Molekulargewicht: 68 kDa

NCBI Accession: NP_001122

UniProt: Q13153

Pathways: MAPK Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Embryonic Body Morphogenesis