Aurora A Antikörper (pThr288)

Produktnummer ABIN362454

Der Kaninchen Polyklonal Anti-Aurora A-Antikörper wurde für IHC validiert. Er ist geeignet, Aurora A in Proben von Human und Maus zu detektieren.

Kurzübersicht für Aurora A Antikörper (pThr288) (ABIN362454)

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Aurora A Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Produktdetails anti-Aurora A Antikörper

Bindungsspezifität: pThr288

Spezifität: The antibody detects endogenous level of Aurora A only when phosphorylated at threonine 288.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pThr288 (R-T-T (p) -L-M) derived from Human Aurora A. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry: 1:50-1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Details zu Aurora A

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Andere Bezeichnung: Aurora A

Hintergrund: Contributes to the regulation of cell cycle progression. Required for normal mitosis. Associates with the centrosome and the spindle microtubules during mitosis and functions in centrosome maturation, spindle assembly, maintenance of spindle bipolarity, centrosome separation and mitotic checkpoint control. Phosphorylates numerous target proteins, including ARHGEF2, BRCA1, KIF2A, NDEL1, PARD3, PLK1 and BORA. Regulates KIF2A tubulin depolymerase activity By similarity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization.

Molekulargewicht: 48 kDa

NCBI Accession: NP_003591

UniProt: O14965

Pathways: Zellzyklus, Asymmetric Protein Localization