Cytokeratin 18 Antikörper (pSer33)

Produktnummer ABIN362391

Dieses Anti-Cytokeratin 18-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Cytokeratin 18 in WB und IHC. Geeignet für Human.

Kurzübersicht für Cytokeratin 18 Antikörper (pSer33) (ABIN362391)

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cytokeratin 18 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-Cytokeratin 18 Antikörper

Bindungsspezifität: pSer33

Spezifität: The antibody detects endogenous level of Keratin 18 only when phosphorylated at serine 33.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pSer33 (A-A-S (p) -V-Y) derived from Human Keratin 18 (CK18) . Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Details zu Cytokeratin 18

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Andere Bezeichnung: Keratin 18

Hintergrund: KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. utations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.

Molekulargewicht: 46 kDa

NCBI Accession: NP_000215

UniProt: P05783

Pathways: Apoptose, Caspase Kaskade in der Apoptose