KIT Antikörper (pTyr721)

Produktnummer ABIN362294

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch KIT in WB. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.Es wurde in 1 Publikation zitiert.

Kurzübersicht für KIT Antikörper (pTyr721) (ABIN362294)

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser KIT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-KIT Antikörper

Bindungsspezifität: pTyr721

Spezifität: The antibody detects endogenous level of c-Kit only when phosphorylated at tyrosine 721.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pTyr721 (N-E-Y (p) -M-D) derived from Human c-Kit. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:500-1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Referenzen für anti-KIT Antikörper (ABIN362294)

Gao, Lin, Gao, Li, Wang, Yu: "MicroRNA-193b regulates c-Kit proto-oncogene and represses cell proliferation in acute myeloid leukemia." in: Leukemia research, Vol. 35, Issue 9, pp. 1226-32, (2011) (PubMed).

Details zu KIT

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Andere Bezeichnung: c-Kit

Hintergrund: This is the receptor for stem cell factor (mast cell growth factor). It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K)

Molekulargewicht: 145 kDa

NCBI Accession: NP_000213

UniProt: P10721

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity