HSP27 Antikörper

Produktnummer ABIN361756

Dieses Anti-HSP27-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von HSP27 in WB, IHC, IF, FACS, IP und ICC. Geeignet für Human. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für HSP27 Antikörper (ABIN361756)

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HSP27 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Klon: 8A7

Produktdetails anti-HSP27 Antikörper

Spezifität: Detects ~25 kDa or ~27 kDa. Recognizes HSP25 and HSP27, cross reacts with alpha B crystallin.

Kreuzreaktivität: Rind (Kuh), Hund, Meerschweinchen, Hamster, Human, Maus, Ratte, Schaf

Aufreinigung: Protein G Purified

Immunogen: HSP27 peptide

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

• WB (1:5000)
• ICC/IF (1:200)
• IHC (1:100)
• optimal dilutions for assays should be determined by the user.

Kommentare:

A 1:5000 dilution of ABIN361755 was sufficient for detection of HSP27 in 20 μg of HeLa cell lysate by ECL immunoblot analysis.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Referenzen für anti-HSP27 Antikörper (ABIN361756)

Cases, Joseph, Obry, Santin, Ben-Yacoub, Pâques, Amsellem-Levera, Bribian, Simonutti, Augustin, Debeir, Sahel, Christ, de Castro, Lehéricy, Cosette, Kozyraki: "Foxg1-Cre Mediated Lrp2 Inactivation in the Developing Mouse Neural Retina, Ciliary and Retinal Pigment Epithelia Models Congenital High Myopia." in: PLoS ONE, Vol. 10, Issue 6, pp. e0129518, (2016) (PubMed).

Details zu HSP27

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Andere Bezeichnung: HSP27

Hintergrund: HSP25 is the mouse homologue of the human HSP27 protein, a member of the small heat shock protein family comprised of a diverse group of proteins from ~15 to >30 kDa(1). The basic structure of most sHSPs is a homologous and highly conserved amino acid sequence, with an α-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (2, 3). HSP27-oligomers consist of stable dimers formed by as many as 8-40 HSP27 protein monomers (4). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (5). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli relevant to the cell type examined. Thus, HSP27 has been suggested to be an important intermediate in second messenger-mediated signaling pathways (6). Other functions include chaperone activity (as mentioned above), thermo-tolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. HSP27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that HSP27 may serve some role in cross-bridge formation between actin and myosin (7). And finally, HSP27 is also thought to be involved in the process of cell differentiation. The up-regulation of HSP27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that HSP27 may play a crucial role in termination of growth (8). For more information visit our HSP27 Scientific Resource Guide at http://www.HSP27.com.

Gen-ID: 3315

NCBI Accession: NP_001532

Pathways: MAPK Signalweg, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling