Glutamate Receptor 1 Antikörper (pSer831)

Produktnummer ABIN361476

Dieses Anti-Glutamate Receptor 1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Glutamate Receptor 1 in WB. Geeignet für Ratte. Dieses Primary Antibody wurde in 9+ Publikationen zitiert.

Kurzübersicht für Glutamate Receptor 1 Antikörper (pSer831) (ABIN361476)

Target: Glutamate Receptor 1 (GLUR1)

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Glutamate Receptor 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Glutamate Receptor 1 Antikörper

Bindungsspezifität: pSer831

Spezifität: Specific for the ~100k GluR1 protein phosphorylated at Ser831. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Immunolabeling is completely eliminated by λ-phosphatase treatment.

Kreuzreaktivität: Maus, Ratte (Rattus)

Kreuzreaktivität (Details): The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with canine, chicken, human and mouse based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Homologie: canine, chicken, human

Aufreinigung: Antigen Affinity Purified from Pooled Serum

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser831 conjugated to KLH

Anwendungsinformationen

Applikationshinweise: WB: 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Handhabung: Avoid repeated freezing and thawing

Lagerung: -20 °C

Referenzen für anti-Glutamate Receptor 1 Antikörper (ABIN361476)

Mao, Wang: "Upregulation of AMPA receptor GluA1 phosphorylation by blocking adenosine A1 receptors in the male rat forebrain." in: Brain and behavior, Vol. 10, Issue 3, pp. e01543, (2020) (PubMed).

Xue, Chen, He, Jin, Mao, Wang: "Integrated regulation of AMPA glutamate receptor phosphorylation in the striatum by dopamine and acetylcholine." in: Neuropharmacology, (2016) (PubMed).

Pasek, Wang, Colbran: "Differential CaMKII regulation by voltage-gated calcium channels in the striatum." in: Molecular and cellular neurosciences, Vol. 68, pp. 234-43, (2016) (PubMed).

Mao, Xue, Jin, Wang: "Dynamic increases in AMPA receptor phosphorylation in the rat hippocampus in response to amphetamine." in: Journal of neurochemistry, Vol. 133, Issue 6, pp. 795-805, (2015) (PubMed).

Mao, Hastings, Fibuch, Wang: "Propofol selectively alters GluA1 AMPA receptor phosphorylation in the hippocampus but not prefrontal cortex in young and aged mice." in: European journal of pharmacology, Vol. 738, pp. 237-44, (2014) (PubMed).

Xue, Edwards, Mao, Guo, Jin, Fibuch, Wang: "Rapid and sustained GluA1 S845 phosphorylation in synaptic and extrasynaptic locations in the rat forebrain following amphetamine administration." in: Neurochemistry international, Vol. 64, pp. 48-54, (2013) (PubMed).

Ferrario, Loweth, Milovanovic, Wang, Wolf: "Distribution of AMPA receptor subunits and TARPs in synaptic and extrasynaptic membranes of the adult rat nucleus accumbens." in: Neuroscience letters, Vol. 490, Issue 3, pp. 180-4, (2011) (PubMed).

Jiao, Jalan-Sakrikar, Robison, Baucum, Bass, Colbran: "Characterization of a central Ca2+/calmodulin-dependent protein kinase IIalpha/beta binding domain in densin that selectively modulates glutamate receptor subunit phosphorylation." in: The Journal of biological chemistry, Vol. 286, Issue 28, pp. 24806-18, (2011) (PubMed).

Sears, Liu, Narayanan, Sharf, Yeckel, Laubach, Aghajanian, DiLeone: "Regulation of nucleus accumbens activity by the hypothalamic neuropeptide melanin-concentrating hormone." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 24, pp. 8263-73, (2010) (PubMed).

Details zu Glutamate Receptor 1

Target: Glutamate Receptor 1 (GLUR1)

Andere Bezeichnung: GRIA1

Hintergrund: Affinity purified rabbit polyclonal antibody. Biological Significance: The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990; Hollmann and Heinemann, 1994). The GluR1 subunit is widely expressed throughout the nervous system. GluR1 is potentiated by phosphorylation at Ser831 which has been shown to be mediated by either PKC or CaM kinase II (McGlade-McCulloh et al., 1993; Mammen et al., 1999; Roche et al., 1996). In addition, phosphorylation of this site has been linked to synaptic plasticity as well s learning and memory (Soderling and Derkach, 2000).

Molekulargewicht: '100 kDa

Gen-ID: 50592

NCBI Accession: NP_113796

UniProt: P19490

Pathways: PI3K-Akt Signalweg