MAPT Antikörper (pSer416)

Produktnummer ABIN361456

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch MAPT in WB. Er zeigt eine Reaktivität gegenüber Ratte und wurde in 1 Publikation erwähnt.

Kurzübersicht für MAPT Antikörper (pSer416) (ABIN361456)

Target: MAPT (Microtubule-Associated Protein tau (MAPT))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MAPT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-MAPT Antikörper

Bindungsspezifität: pSer416

Spezifität: Specific for ~59, 65, 68k tau protein phosphorylated at Ser416.

Kreuzreaktivität: Maus, Ratte (Rattus)

Homologie: bovine, canine, human, non-human primate

Aufreinigung: Antigen Affinity Purified from Pooled Serum

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser416 conjugated to KLH

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Lagerung: -20 °C

Referenzen für anti-MAPT Antikörper (ABIN361456)

Wang, Hall, Lee, Kamgar-Parsi, Bi, Baudry: "The tyrosine phosphatase PTPN13/FAP-1 links calpain-2, TBI and tau tyrosine phosphorylation." in: Scientific reports, Vol. 7, Issue 1, pp. 11771, (2019) (PubMed).

Details zu MAPT

Target: MAPT (Microtubule-Associated Protein tau (MAPT))

Andere Bezeichnung: MAPT

Hintergrund: Tau is a key microtubule-associated protein that plays an important role in the formation of microtubules in axons (Binder et al. 1985). Six tau isoforms have been identified as products of a single gene produced by alternative mRNA splicing (Goedert 1990). Tau mutations have been implicated in many neurodegenerative disorders such as Alzheimer’s disease (AD), Pick’s disease and progressive supranuclear palsy. It has been well documented that hyperphosphorylated tau is a major component of paired helical filaments in AD brain (Lee 1995). Serine 416 has been demonstrated to be a major phosphorylation site in vitro by CaM kinase II (Steiner at al. 1990). Anti-Phospho-Ser416 Tau Western blot of rat brain homogenate showing specific immunolabeling of the ~59, 65, 68k Tau isoforms phosphorylated at Ser416(control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).

Molekulargewicht: 59/65/68 kDa

Gen-ID: 69329

UniProt: P19332

Pathways: MAPK Signalweg, Microtubule Dynamics, M Phase, Regulation of Cell Size