Tryptophan Hydroxylase 1 Antikörper

Produktnummer ABIN361412

Der Schaf Polyklonal Anti-Tryptophan Hydroxylase 1-Antikörper wurde für WB und IHC validiert. Er ist geeignet, Tryptophan Hydroxylase 1 in Proben von Kaninchen zu detektieren.

Kurzübersicht für Tryptophan Hydroxylase 1 Antikörper (ABIN361412)

Target: Tryptophan Hydroxylase 1 (TPH1)

Reaktivität: Kaninchen

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser Tryptophan Hydroxylase 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-Tryptophan Hydroxylase 1 Antikörper

Spezifität: Specific for the ~55k tryptophan hydroxylase protein.

Kreuzreaktivität: Human, Ratte (Rattus)

Homologie: wide variety mammalian

Aufreinigung: Antigen Affinity Purified from Pooled Serum

Immunogen: Recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: WB: 1:1000 IHC (frozen sections, Haycock et al., 2002): 1:1000 Quality Control: Western blots performed on each lot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Lagerung: -20 °C

Details zu Tryptophan Hydroxylase 1

Target: Tryptophan Hydroxylase 1 (TPH1)

Andere Bezeichnung: TPH1

Hintergrund: Tryptophan hydroxylase (TPH) catalyzes the first step in the biosynthesis of serotonin and melatonin (Martinez et al., 2001). Thus, expression of TPH can be used as an indicator of the localization of serotonin and melatonin in brain. In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland (Haycock et al., 2002) . Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis (Martinez et al., 2001). Anti-Tryptophan Hydroxylase Western blot of human dorsal Raphe nucleus showing specifc immunolabeling of the ~55k tryptophan hydroxylase protein.

Molekulargewicht: '55 kDa

Gen-ID: 100101558

UniProt: P17290

Pathways: Feeding Behaviour