NMDAR2A Antikörper (C-Term)

Produktnummer ABIN361396

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch NMDAR2A in WB, IHC und IP. Er zeigt eine Reaktivität gegenüber Ratte und wurde in 1 Publikation erwähnt.

Kurzübersicht für NMDAR2A Antikörper (C-Term) (ABIN361396)

Target: NMDAR2A (GRIN2A) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NMDAR2A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Produktdetails anti-NMDAR2A Antikörper

Bindungsspezifität: C-Term

Spezifität: Specific for the ~180k NR2A subunit of the NMDA receptor. Recognizes human, mouse and rat forms of the NR2A subunit of NMDAR. No reactivity towards the NR2B and NR2C subunits. Immunolabeling is blocked by pre-adsorption of antibody with the fusion protein used to generate the antibody.

Kreuzreaktivität: Human, Maus, Ratte (Rattus)

Aufreinigung: Antigen Affinity Purified from Pooled Serum

Immunogen: Fusion protein from the C-terminal region of the NR2A subunit

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: WB: 1:1000 IHC (frozen sections, unpublished observations): 1:1000 to 1:2000 IP: 3 µl per 200 µg lysate Quality Control: Western blots performed on each lot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized

Lagerung: -20 °C

Referenzen für anti-NMDAR2A Antikörper (ABIN361396)

Morimura, Yasuda, Yamaguchi, Katayama, Hatayama, Tomioka, Odagawa, Kamiya, Iwayama, Maekawa, Nakamura, Matsuzaki, Tsujii, Yamada, Yoshikawa, Aruga: "Autism-like behaviours and enhanced memory formation and synaptic plasticity in Lrfn2/SALM1-deficient mice." in: Nature communications, Vol. 8, pp. 15800, (2018) (PubMed).

Details zu NMDAR2A

Target: NMDAR2A (GRIN2A) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A))

Andere Bezeichnung: GRIN2A

Hintergrund: The ion channels activated by glutamate are typically divided into two classes. Glutamate receptors that are activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002, Wenthold et al., 2003, Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989, Alvestad et al., 2003, Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits. Anti-NMDA Receptor, NR2A Subunit Western blot of 10 ug of rat hippocampal (Hipp) lysate showing specific immunolabeling of the ~180k NR2A subunit of the NMDA receptor.

Molekulargewicht: '180 kDa

Gen-ID: 24409

UniProt: Q00959

Pathways: Synaptic Membrane, Regulation of long-term Neuronal Synaptic Plasticity