GFAP Antikörper

Produktnummer ABIN361379

Der Huhn Polyklonal Anti-GFAP-Antikörper wurde für WB und IF validiert. Er ist geeignet, GFAP in Proben von Rind (Kuh) zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für GFAP Antikörper (ABIN361379)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reaktivität: Rind (Kuh)

Wirt: Huhn

Klonalität: Polyklonal

Konjugat: Dieser GFAP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Produktdetails anti-GFAP Antikörper

Spezifität: Specific for the ~50kDa GFAP protein. A lower band at ~45kDa is a proteolytic fragment derived from the GFAP molecule. It is expected that the antibody will react with other mammalian tissues.

Kreuzreaktivität: Ratte (Rattus)

Homologie: human, mouse

Aufreinigung: Total IgY fraction

Immunogen: Recombinant and purified bovine GFAP

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: WB: 1:10,000 IF: 1:1,000 Quality Control: Western blots performed on each lot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: total IgY fraction in PBS + 10 mM Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Referenzen für anti-GFAP Antikörper (ABIN361379)

Levenga, Wong, Milstead, Keller, LaPlante, Hoeffer: "AKT isoforms have distinct hippocampal expression and roles in synaptic plasticity." in: eLife, Vol. 6, (2018) (PubMed).

Details zu GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Andere Bezeichnung: GFAP

Hintergrund: Glial Fibrillary Acidic Protein (GFAP) was discovered by Amico Bignami and co-workers as a major fibrous protein of multiple sclerosis plaques (1). It was subsequently found to be a member of the 10nm or intermediate filament (IF) family, specifically the IF family Class III, which also includes peripherin, desmin and vimentin. GFAP is strongly and specifically expressed in astrocytes and certain other astroglia in the CNS, in satellite cells, peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In many damage and disease states GFAP expression is heavily upregulated in astrocytes. In addition, neural stem cells frequently strongly express GFAP. Point mutations in the protein coding region of the GFAP gene lead to Alexander disease which is characterized by the presence of abnormal astrocytes containing GFAP protein aggregates known as Rosenthal fibers (2). Anti-GFAP Left: Western blot of rat cortex lysate showing specific immunolabeling of the ~ 50k GFAP protein. Right: Mixed cultures of neurons and glia stained with chicken anti-GFAP (red), and DNA (blue). Astrocytes stain strongly and specifically in a clearly filamentous fashion with this antibody.

Molekulargewicht: '50 kDa

Gen-ID: 281189

UniProt: Q28115