SMURF1 Antikörper (C-Term)

Produktnummer ABIN357470

Der Kaninchen Polyklonal anti-SMURF1 Antikörper wird verwendet zum Nachweis von SMURF1 in Proben von Human und Maus. Er wurde validiert für WB, IHC (p) und EIA.

Kurzübersicht für SMURF1 Antikörper (C-Term) (ABIN357470)

Target: SMURF1 (SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SMURF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-SMURF1 Antikörper

Bindungsspezifität: C-Term

Spezifität: This antibody detects SMURF1 at C-term.

Aufreinigung: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of SMURF1.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunohistochemistry 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Details zu SMURF1

Target: SMURF1 (SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1))

Andere Bezeichnung: SMURF1

Hintergrund: SMURF1 is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. This protein interacts with receptor-regulated SMADs specific for the BMP pathway, SMAD1 and SMAD5, in order to trigger their ubiquitination and degradation and thereby their inactivation.Synonyms: E3 ubiquitin-protein ligase SMURF1, KIAA1625, SMAD ubiquitination regulatory factor 1, SMAD-specific E3 ubiquitin-protein ligase 1, SMURF 1, SMURF-1

Molekulargewicht: 86113 Da

Gen-ID: 57154, 9606

UniProt: Q9HCE7

Pathways: Regulation of Cell Size