DKK3 Antikörper (N-Term)

Produktnummer ABIN357071

Der Kaninchen Polyklonal Anti-DKK3-Antikörper wurde für WB, IHC (p) und EIA validiert. Er ist geeignet, DKK3 in Proben von Human zu detektieren.

Kurzübersicht für DKK3 Antikörper (N-Term) (ABIN357071)

Target: DKK3

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DKK3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-DKK3 Antikörper

Bindungsspezifität: N-Term

Spezifität: This antibody detects DKK3 at N-term.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Protein G Chromatography eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human DKK3.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu DKK3

Target: DKK3

Andere Bezeichnung: Dickkopf-3 (DKK3)

Hintergrund: DKK3, like DKK1, DKK2, and DKK4, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis revealed wide expression of the DKK3 transcript, with highest expression in heart, brain, and spinal cord. In situ hybridization reveals highest expression in mouse brain, eye, and heart.Synonyms: Dickkopf-related protein 3, Dkk-3, REIC

Molekulargewicht: 38291 Da

Gen-ID: 27122

NCBI Accession: NP_001018067

UniProt: Q9UBP4