GST Antikörper
Kurzübersicht für GST Antikörper (ABIN356807)
Target
Alle GST Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
-
-
Aufreinigung
- Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
-
Immunogen
- This antibody is generated from rabbits immunized with a recombinant full length of GST.
-
Isotyp
- Ig Fraction
-
-
-
-
Applikationshinweise
-
ELISA: 1/1,000. Western blot: 1/100-1/500Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
-
-
Format
- Liquid
-
Konzentration
- 0.25 mg/mL
-
Buffer
- PBS containing 0.09 % (W/V) Sodium Azide as preservative.
-
Konservierungsmittel
- Sodium azide
-
Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Handhabung
- Avoid repeated freezing and thawing.
-
Lagerung
- 4 °C/-20 °C
-
Informationen zur Lagerung
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
-
-
- GST (Glutathione S Transferase (GST))
-
Andere Bezeichnung
- Glutathione-S-Transferase (GST)
-
Hintergrund
- Glutathione S-transferase (GST) was originally cloned from parasite Schistosoma japonicum and it is now a widely used protein fusion partner. Vectors containing GST Tag have been developed for both prokaryotic and eukaryotic systems. The GST fusion proteins are easily purified from cell lysates by affinity chromatography using Glutathione Sepharose 4B to elute out the GST fusion protein from the column with a denaturing form of glutathione. Using anti-GST antibody provides a simple solution to detect the expression of GST fusion proteins in cells.Synonyms: GST26-Tag, Glutathione S-transferase Tag
-
Gen-ID
- 6182
-
UniProt
- P08515
Target
-