PIAS2 Antikörper (C-Term)

Produktnummer ABIN356767

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch PIAS2 in WB, IHC (p) und EIA. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für PIAS2 Antikörper (C-Term) (ABIN356767)

Target: PIAS2 (Protein Inhibitor of Activated STAT, 2 (PIAS2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PIAS2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-PIAS2 Antikörper

Bindungsspezifität: C-Term

Spezifität: This antibody is specific to PIAS2 (PIASx1/2) (C-term).

Aufreinigung: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PIASx1/2.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu PIAS2

Target: PIAS2 (Protein Inhibitor of Activated STAT, 2 (PIAS2))

Andere Bezeichnung: PIAS2

Hintergrund: PIASX functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. This protein plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. PIASX binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha.Synonyms: ARIP3, Androgen receptor-interacting protein 3, DAB2-interacting protein, DIP, E3 SUMO-protein ligase PIAS2, Miz1, Msx-interacting zinc finger protein, PIAS-NY protein, PIASX, Protein inhibitor of activated STAT x, Protein inhibitor of activated STAT2

Gen-ID: 9063, 9606

UniProt: O75928

Pathways: JAK-STAT Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling