GFP Antikörper (Green Fluorescent Protein)

Details for Product anti-GFP Antibody No. ABIN356346, Anbieter: Anmelden zum Anzeigen
Antigen
  • green fluorescent protein
  • gfp
Reaktivität
Aequorea victoria
637
18
5
5
2
2
1
1
Wirt
Maus
335
176
80
43
16
9
9
2
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser GFP Antikörper ist unkonjugiert
34
31
28
14
13
12
12
10
9
9
8
8
7
6
6
6
6
6
5
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Applikation
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
539
359
203
163
140
136
97
86
75
24
15
13
12
8
7
6
6
5
4
4
3
3
3
3
3
2
2
2
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Purified His-tagged GFP protein was used to produced this monoclonal antibody.
Klon 168AT1211
Isotyp IgG1
Produktmerkmale Synonyms: Green fluorescent protein, GFP-Tag
Reinigung Protein G Chromatography eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Andere Bezeichnung GFP
Substanzklasse Tag
Hintergrund Green fluorescent protein (GFP), originally isolated from the jellyfish Aequorea victoria, is one of the best visual reporters for monitoring gene expression in vivo and in situ. GFP is a also convenient marker for use in flow cytometry because it eliminates the need to incubate with asecondary reagent (such as dyes or antibodies) for detection. However, anti-GFP antibody is also widely used for co-immunoprecipitation, co-localization or western blotting for the confirmation of specificity when a GFP fusion protein is expressed in cells. This monoclonal antibody provides a simple solution to detect the expression of a GFP-tagged protein in cells. Because of its ability to spontaneously generate its own fluorophore, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria is used extensively as a fluorescent marker in molecular and cell biology. The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date. This shift in the spectrum is the result of a T203Y substitution (single-letter amino acid code), a mutation rationally designed on the basis of the X-ray structure of GFP S65T. Anti-GFP monoclonal antibody can detect both GFP and YFP but not BFP (Blue fluorescent protein) by western blotting.Synonyms: GFP-Tag, Green fluorescent protein
UniProt P42212
Applikationshinweise ELISA: 1/3,000. Western blotting: 1/2,000. Immunohistochemistry: 1/50-1/100. Immunofluorescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.25 mg/mL
Buffer PBS containing 0.09 % (w/v) Sodium Azide as preservative.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Haltbarkeit 12 months
Bilder des Herstellers
Western Blotting (WB) image for anti-Green Fluorescent Protein (GFP) antibody (ABIN356346) anti-Green Fluorescent Protein (GFP) antibody
Immunofluorescence (IF) image for anti-Green Fluorescent Protein (GFP) antibody (ABIN356346) anti-Green Fluorescent Protein (GFP) antibody (Image 2)
Produkt verwendet in: Clark, Jovov, Rooj, Fuller, Benos: "Proteolytic cleavage of human acid-sensing ion channel 1 by the serine protease matriptase." in: The Journal of biological chemistry, Vol. 285, Issue 35, pp. 27130-43, 2010 (PubMed).

Maier, Ogihara, Trace, Tersey, Robbins, Chakrabarti, Nunemaker, Stull, Taylor, Thompson, Dondero, Lewis, Dinarello, Nadler, Mirmira: "The unique hypusine modification of eIF5A promotes islet beta cell inflammation and dysfunction in mice." in: The Journal of clinical investigation, Vol. 120, Issue 6, pp. 2156-70, 2010 (PubMed).

Kim, Nadal, Clemens, Baron, Jung, Misumi, Rudy, Hoffman: "Kv4 accessory protein DPPX (DPP6) is a critical regulator of membrane excitability in hippocampal CA1 pyramidal neurons." in: Journal of neurophysiology, Vol. 100, Issue 4, pp. 1835-47, 2008 (PubMed).

Witola, Pessi, El Bissati, Reynolds, Mamoun: "Localization of the phosphoethanolamine methyltransferase of the human malaria parasite Plasmodium falciparum to the Golgi apparatus." in: The Journal of biological chemistry, Vol. 281, Issue 30, pp. 21305-11, 2006 (PubMed).

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