Keratin Acidic (AE1) Antikörper

Produktnummer ABIN356302

Der Maus Monoklonal Anti--Antikörper wurde für IF, IHC (p) und IHC (fro) validiert. Er ist geeignet, in Proben von Human, Ratte, Huhn, Rind (Kuh), Maus, Kaninchen, Affe und Schwein zu detektieren.

Kurzübersicht für Keratin Acidic (AE1) Antikörper (ABIN356302)

Target: Keratin Acidic (AE1)

Reaktivität: Human, Ratte, Huhn, Rind (Kuh), Maus, Kaninchen, Affe, Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: AE1

Produktdetails

Spezifität: This antibody recognizes low molecular weight cytokeratins (CK 10 (56.5), CK14 (50), CK15 (50), CK16 (48) and CK19(40) of the acidic family.
Cellular Localization: Cytoplasmic.

Produktmerkmale: Synonyms: LMW Cytokeratins, Cytokeratin type I, Keratin type I

Aufreinigung: Protein A Chromatography.

Immunogen: Human epithelial keratin. Remarks: Molecular weight of antigen: (CK 10 (56. 5), CK14 (50), CK15 (50), CK16 (48) andCK19 (40).

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears). For IHC dilute concentared antibody at 1/50-1/100, use streptavidinapprox. biotin system orpolymer system, incubate 30 minutes at room temperature. FFPE tissue section requires antigen retrieval (boiling tissue in 10 mM citrate, pH 6.0 for10-15 minutes followed by cooling for 10-15 minutes). Recommended Positive Control: Human skin, squamous cell Ca.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS, pH 7.4 containing 1 % BSA as stabilizer and 0.05 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Haltbarkeit: 12 months

Antigendetails

Target: Keratin Acidic (AE1)

Hintergrund: Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1, CK2, CK3, CK4, CK5, CK6, CK7, CK8 and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10, CK12, CK 13, CK14, CK16, CK17, CK18, CK19 and CK20.Synonyms: Cytokeratin type I, Keratin type I, LMW Cytokeratins