CD3 Antikörper

Produktnummer ABIN356219

Produktdetails anti-CD3 Antikörper

Target: CD3

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD3 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Functional Studies (Func)

Spezifität: This Low Endotoxin/Azide free antibody is specific to CD3e (Mr. 25 kDa). The monoclonal antibody C363.29B recognizes an epitope on the 25 kDa epsilon chain of the CD3/TCR complex. (Ref.1) In the presence of Fc receptor-bearing accessory cells, soluble C363.29B can activate primed and naive T cell in vitro. Immobilized C363.29 monoclonal antibody can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present. (Ref.1,3,4) The monoclonal antibody is cytolytic, easily used for cell surface staining and a good immunoprecipitating antibody. (Ref.1)

Produktmerkmale: Synonyms: T3/Leu-4, T-cell surface glycoprotein CD3, T-cell surface antigen T3/Leu-4

Endotoxin-Niveau: Low Endotoxin

Klon: C363-29B (YCD3-1)

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: Identification and enumeration of CD3+. (Ref.1,2). Functional Assay: In vitro Depletion of CD3+ cells. In vitro Activation of T cells (accessory cells must be provided). (Ref.1,3,4)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: PBS without preservatives.

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu CD3

Target: CD3

Andere Bezeichnung: CD3 (CD3 Produkte)

Hintergrund: CD3e, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chain ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains. (Ref.1,2) CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4

Gen-ID: 12501

UniProt: P22646

Pathways: T-Zell Rezeptor Signalweg, Ubiquitin Proteasome Pathway