GFAP Antikörper

Produktnummer ABIN351523

Dieses Maus Monoklonal-Antikörper erkennt spezifisch GFAP in WB, IHC, IF, IHC (p) und IP. Er zeigt eine Reaktivität gegenüber Human, Ratte, Schwein und Huhn.

Kurzübersicht für GFAP Antikörper (ABIN351523)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reaktivität: Human, Ratte, Schwein, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GFAP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Produktdetails anti-GFAP Antikörper

Marke: IHC-plus™

Spezifität: Recognizes porcine glial fibrillary acidic protein (GFAP) at 51-52kD. Species cross-reactivity: human, rat and chicken.

Aufreinigung: Protein G purified

Immunogen: Porcine spinal cord. Cellular Localization: Cytoplasmic.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: IF, IHC, IHC-P (1 - 2 μg/mL), IP (2 μg/tst), WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1-2 μg/mL. Immunoprecipitation (Native and denatured): 2 μg/mg protein lysate (Use Protein G). Western Blot: 1-2 μg/mL for 2 hrs at RT. Positive control: Astrocytoma, IMR5 and brain cells.

Kommentare:

Target Species of Antibody: Pig

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.1 % sodium azide, 40 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Andere Bezeichnung: GFAP

Hintergrund: Name/Gene ID: GFAP

Synonyms: GFAP

Gen-ID: 2670

UniProt: P14136