ARPC1B Antikörper (AA 2-14)

Produktnummer ABIN351466

Produktdetails anti-ARPC1B Antikörper

Target: ARPC1B (Actin Related Protein 2/3 Complex, Subunit 1B, 41kDa (ARPC1B))

Bindungsspezifität: AA 2-14

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Pferd, Schwein, Kaninchen, Affe, Hamster

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ARPC1B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human ARPC1B / p41-ARC.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Horse, Rabbit, Pig (100%) Bat, Opossum, Turkey, Chicken, Platypus, Catfish, Salmon, Sablefish, Stickleback, Pufferfish, Zebrafish (92%) Xenopus (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide AYHSFLVEPISCH from the N-terminus of human ARPC1B / p41-ARC (NP_005711.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Horse, Rabbit, Pig (100%), Bat, Opossum, Turkey, Chicken, Platypus, Catfish, Salmon, Sablefish, Stickleback, Pufferfish, Zebrafish (92%), Xenopus (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:32000), WB (2 - 4 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu ARPC1B

Target: ARPC1B (Actin Related Protein 2/3 Complex, Subunit 1B, 41kDa (ARPC1B))

Andere Bezeichnung: ARPC1B / p41-ARC / ARP2 (ARPC1B Produkte)

Hintergrund: Name/Gene ID: ARPC1B

Synonyms: ARPC1B, ARC41, Arp2/3 complex 41 kd subunit, Arp2/3 complex 41 kDa subunit, p41-ARC, p40-ARC

Gen-ID: 10095

NCBI Accession: NP_005711

UniProt: O15143

Pathways: RTK Signalweg, Regulation of Actin Filament Polymerization