NEDD8 Antikörper (N-Term)

Produktnummer ABIN350514

Dieses Anti-NEDD8-Antikörper ist ein Schaf Polyklonal-Antikörper zur Detektion von NEDD8 in WB, IHC und IHC (p). Geeignet für Maus.

Kurzübersicht für NEDD8 Antikörper (N-Term) (ABIN350514)

Target: NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))

Reaktivität: Maus

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser NEDD8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-NEDD8 Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: Sheep antibody to NEDD8

Spezifität: Specific for NEDD8.

Kreuzreaktivität: Human, Maus, Ratte

Kreuzreaktivität (Details): Other species not yet tested.

Aufreinigung: IgG

Immunogen: A synthetic peptide from the n-terminal of mouse NEDD8 (Ubiquitin-like protein Nedd8 Neddylin) conjugated to immunogenic carrier protein has been used as the immunogen. The antigen is homologous in human rat zebrafish drosophila and xenopus.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC (both on frozen,paraffin embedded tissues) WB. Use at a concentration of 10-50,micro,g,ml. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

Handhabung: Avoid freeze and thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Haltbarkeit: 12 months

Details zu NEDD8

Target: NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))

Andere Bezeichnung: NEDD8

Hintergrund: Function: Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory protein. Subcellular location: Mainly nuclear Tissue specificity: Ubiquitously expressed (at protein level). Post-translational modification: Cleavage of precursor form by UCHL3 or SENP8 is necessary for function

UniProt: P29595

Pathways: Ubiquitin Proteasome Pathway