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GFP Antikörper

GFP Reaktivität: Aequorea victoria WB, IHC Wirt: Kaninchen Polyclonal unconjugated

Produktnummer ABIN350304

Datenblatt (PDF)

Target Alle GFP Antikörper anzeigen

GFP (Green Fluorescent Protein (GFP))
Reaktivität
164

14

11

9

9

5

5

5
Aequorea victoria
Wirt
67

61

35

14

5

5

4

2

1
Kaninchen
Passende Sekundärantikörper
Klonalität
111

79
Polyklonal
Konjugat
86

11

10

10

5

4

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser GFP Antikörper ist unkonjugiert
Applikation
148

81

61

45

32

30

30

23

16

13

12

12

12

9

4

4

2

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC)

Verwendungszweck

Rabbit antibody to EGFP/GFP/Venus

Spezifität

Specific for EGFP/GFP/Venus

Kreuzreaktivität (Details)

Any organism expressing EGFP/GFP/Venus.

Aufreinigung

IgG

Immunogen

Recombinant EGFP/GFP/Venus protein was used as the antigen.

Isotyp

IgG

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GFP Reaktivität: Aequorea victoria WB, IHC, FM, DB, FLISA Wirt: Ziege Polyclonal FITC

Applikationshinweise

IHC WB. A working concentration of 5-50 μg,ml is recommended. The optimal concentration should be determined by the end user.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Lyophilized

Rekonstitution

Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Handhabung

Avoid freeze and thaw cycles.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term.

Haltbarkeit

12 months
Target

GFP (Green Fluorescent Protein (GFP))

Abstract

GFP Produkte

Hintergrund

GFP is a naturally fluorescent protein discovered by chance in the 1960s by Shimomura et al. GFP makes green light out of aequorin's blue light. The chromophore is a modified Ser-Tyr-Gly sequence. GFP can emit light once this short tripeptide has adopted a cyclic conformation with the help of a oxygen. BIOPHYSICOCHEMICAL PROPERTIES: Excitation max (nm): 488; Emission max (nm): 509; Extinction coefficient (Cm-1M-1): 61000. Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer reporter of gene expression or as a measure of protein-protein interactions.