GFP Antikörper

Produktnummer ABIN350304

Dieser Anti-GFP-Antikörper ist ein Kaninchen-Polyklonal-Antikörper zum Nachweis von GFP in WB und IHC. Geeignet für Aequorea victoria.

Kurzübersicht für GFP Antikörper (ABIN350304)

Target: GFP (Green Fluorescent Protein (GFP))

Reaktivität: Aequorea victoria

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GFP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-GFP Antikörper

Verwendungszweck: Rabbit antibody to EGFP/GFP/Venus

Spezifität: Specific for EGFP/GFP/Venus

Kreuzreaktivität (Details): Any organism expressing EGFP/GFP/Venus.

Aufreinigung: IgG

Immunogen: Recombinant EGFP/GFP/Venus protein was used as the antigen.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC WB. A working concentration of 5-50 μg,ml is recommended. The optimal concentration should be determined by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Handhabung: Avoid freeze and thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term.

Haltbarkeit: 12 months

Details zu GFP

Target: GFP (Green Fluorescent Protein (GFP))

Hintergrund: GFP is a naturally fluorescent protein discovered by chance in the 1960s by Shimomura et al. GFP makes green light out of  aequorin's blue light. The chromophore is a modified Ser-Tyr-Gly sequence. GFP can emit light once this short tripeptide has adopted a cyclic conformation with the help of a oxygen. BIOPHYSICOCHEMICAL PROPERTIES: Excitation max (nm): 488; Emission max (nm): 509; Extinction coefficient (Cm-1M-1): 61000. Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer reporter of gene expression or as a measure of protein-protein interactions.