GFP Antikörper

Produktnummer ABIN350302

Dieses Schaf Polyklonal-Antikörper erkennt spezifisch GFP in WB und IHC. Er zeigt eine Reaktivität gegenüber Aequorea victoria und wurde in 1 Publikation erwähnt.

Kurzübersicht für GFP Antikörper (ABIN350302)

Target: GFP (Green Fluorescent Protein (GFP))

Reaktivität: Aequorea victoria

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser GFP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-GFP Antikörper

Verwendungszweck: Sheep antibody to EGFP/GFP/Venus

Spezifität: Specific for EGFP/GFP/Venus

Kreuzreaktivität (Details): Any organism expressing EGFP/GFP/Venus.

Aufreinigung: IgG

Immunogen: Recombinant EGFP/GFP/Venus protein was used as the antigen.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC WB. Use at a concentration of 10-50 μg,ml. The optimal working concentration should be determined by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Handhabung: Avoid freeze and thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-GFP Antikörper (ABIN350302)

Choi, Kim, Song, Cha, Cho, Son, Kim, Mook-Jung: "Amyloid β-induced elevation of O-GlcNAcylated c-Fos promotes neuronal cell death." in: Aging cell, Vol. 18, Issue 1, pp. e12872, (2020) (PubMed).

Details zu GFP

Target: GFP (Green Fluorescent Protein (GFP))

Hintergrund: GFP is anaturally fluorescent protein discovered by chance in the 1960s by Shimomura et al. BIOPHYSICOCHEMICAL PROPERTIES: Excitation max (nm): 488, Emission max (nm): 509, Extinction coefficient (Cm-1M-1): 61000. Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins.