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Keratin 5/8 Antikörper (AA 353-367) (Biotin)

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Keratin 5/8 in FACS, IHC, IHC (p), IHC (fro) und ELISA. Er zeigt eine Reaktivität gegenüber Human.
Produktnummer ABIN344035

Kurzübersicht für Keratin 5/8 Antikörper (AA 353-367) (Biotin) (ABIN344035)

Target

Keratin 5/8 (KRT5/8)

Reaktivität

  • 23
  • 17
  • 16
  • 14
  • 14
  • 14
  • 13
  • 11
  • 10
  • 3
  • 2
  • 2
Human

Wirt

  • 23
Maus

Klonalität

  • 23
Monoklonal

Konjugat

  • 10
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser Keratin 5/8 Antikörper ist konjugiert mit Biotin

Applikation

  • 17
  • 15
  • 13
  • 13
  • 8
  • 8
  • 5
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA
  • Bindungsspezifität

    AA 353-367

    Spezifität

    Reactivities on Cultured MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650. Cell lines (tested so far) SSC-12, bovine BMGE+H, BMGE-H, MDBK. Represents an excellent marker for distinguishing carcinomas from all non-epithelial tumors. Specifically reacts with cytokeratins 5 and 8 present in nearly all epithelia. Polypeptide reacting: Mr 52500, Mr 58000 cytokeratins (type II cytokeratins nos. 5+8) of human epithelial cells. Epitope has been mapped to aa353-367 on alpha helical rod domain. Species cross-reactivity: Human, bovine, murine, amphibia, porcine, hydra.

    Aufreinigung

    Protein A purified

    Immunogen

    Human cytokeratin 8, purified from SDS PAGE.

    Type of Immunogen: Purified protein

    Isotyp

    IgG1
  • Applikationshinweise

    Approved: ELISA, Flo, IHC, IHC-Fr, IHC-P

    Usage: Suitable for use in ELISA, FACS and Immunohistochemistry (frozen and paraffin-embedded tissue and cytological material, Pretreatment With paraffin embedded sections, protease pretreatment is required prior to antibody application). Immunohistochemistry: 1:10-1:20, Incubation Time 1hr at RT, extended with paraffin sections (overnight at 2-8?C). The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

    Kommentare

    Target Species of Antibody: Human

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    Lot specific
  • Target

    Keratin 5/8 (KRT5/8)

    Andere Bezeichnung

    Cytokeratin 5+8

    Hintergrund

    This antibody detects: Cytokeratin 5+8
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