Keratin 5/8 Antikörper (AA 353-367) (Biotin)
Kurzübersicht für Keratin 5/8 Antikörper (AA 353-367) (Biotin) (ABIN344035)
Target
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
-
-
Bindungsspezifität
- AA 353-367
-
Spezifität
- Reactivities on Cultured MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650. Cell lines (tested so far) SSC-12, bovine BMGE+H, BMGE-H, MDBK. Represents an excellent marker for distinguishing carcinomas from all non-epithelial tumors. Specifically reacts with cytokeratins 5 and 8 present in nearly all epithelia. Polypeptide reacting: Mr 52500, Mr 58000 cytokeratins (type II cytokeratins nos. 5+8) of human epithelial cells. Epitope has been mapped to aa353-367 on alpha helical rod domain. Species cross-reactivity: Human, bovine, murine, amphibia, porcine, hydra.
-
Aufreinigung
- Protein A purified
-
Immunogen
-
Human cytokeratin 8, purified from SDS PAGE.
Type of Immunogen: Purified protein -
Isotyp
- IgG1
-
-
-
-
Applikationshinweise
-
Approved: ELISA, Flo, IHC, IHC-Fr, IHC-P
Usage: Suitable for use in ELISA, FACS and Immunohistochemistry (frozen and paraffin-embedded tissue and cytological material, Pretreatment With paraffin embedded sections, protease pretreatment is required prior to antibody application). Immunohistochemistry: 1:10-1:20, Incubation Time 1hr at RT, extended with paraffin sections (overnight at 2-8?C). The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
Kommentare
-
Target Species of Antibody: Human
-
Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
-
-
Konzentration
- Lot specific
-
-
- Keratin 5/8 (KRT5/8)
-
Andere Bezeichnung
- Cytokeratin 5+8
-
Hintergrund
- This antibody detects: Cytokeratin 5+8
Target
-