EGFR Antikörper (pTyr1173)

Produktnummer ABIN343717

Dieses Maus Monoklonal-Antikörper erkennt spezifisch EGFR in WB, IP und ICFC. Er zeigt eine Reaktivität gegenüber Human und wurde in 1 Publikation erwähnt.

Kurzübersicht für EGFR Antikörper (pTyr1173) (ABIN343717)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EGFR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Intracellular Flow Cytometry (ICFC)

Klon: EM-13

Produktdetails anti-EGFR Antikörper

Bindungsspezifität: pTyr1173

Verwendungszweck: Anti-Hu EGFR (pY1173) Purified

Spezifität: The mouse monoclonal antibody EM-13 reacts with human EGFR (ErbB1 / HER1) phosphorylated on intracellular tyrosine 1173.

Kreuzreaktivität (Details): Human

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Synthetic phospho-peptide covering sequence around tyrosine 1173 of human EGFR.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western blotting: Recommended dilution: 1 μg/mL, positive control: EGF-stimulated A431, negative control: non-stimulated A431.
Flow cytometry: Recommended dilution: 1-4 μg/mL. Intracellular staining.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-EGFR Antikörper (ABIN343717)

Shlepova, Bychkov, Shipunova, Shramova, Shulepko, Gornostaeva, Kiseleva, Kukushkin, Kazakov, Tukhovskaya, Dyachenko, Murashev, Shenkarev, Deyev, Kirpichnikov, Lyukmanova: "Combination with a Low Dose of Doxorubicin Further Boosts the Antitumor Effect of SLURP-1 In Vivo and Associates with EGFR Down-Regulation." in: Acta naturae, Vol. 17, Issue 1, pp. 87-96, (2025) (PubMed).

Details zu EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Andere Bezeichnung: EGFR

Hintergrund: Epidermal growth factor receptor,The oncoprotein EGFR (epidermal growth factor receptor), also known as HER1 / ErbB1, is a member of ErbB family of cell surface receptor tyrosine kinases. This 170 kDa transmembrane glycoprotein is often associated with cancerogenesis, although its activation state is controlled at various levels including trafficking and degradation steps. Binding of receptor-specific ligands to the EGFR ectodomain results in formation of homodimeric or heterodimeric kinase-active complexes into which HER2 / ErbB2 is recruited as a preferred partner. Subsequent signaling cascades such as RAS/MAPK and PI3K/AKT pathways lead to cell proliferation and survival responses.,EGFR, EGF-R, Her1, ERBB1, PIG61, NISBD2

Gen-ID: 1956

UniProt: P00533

Pathways: NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteine