IRF3 Antikörper

Produktnummer ABIN337252

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch IRF3 in WB, IHC und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human und Affe.

Kurzübersicht für IRF3 Antikörper (ABIN337252)

Target: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Reaktivität: Human, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IRF3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-IRF3 Antikörper

Spezifität: Detects endogenous levels of human IRF3 at 51kD mouse and rat, at 57kD human. Species cross-reactivity: mouse, rat and monkey.

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide (KLH-coupled) corresponding to a region around residue 300 of IRF3.

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (1:50), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:50.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 10 mM sodium HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu IRF3

Target: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Andere Bezeichnung: IRF3 / IRF-3

Hintergrund: Name/Gene ID: IRF3

Synonyms: IRF3, Interferon regulatory factor 3, IRF-3

Gen-ID: 3661

UniProt: Q14653

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades