CTNNB1 Antikörper (C-Term)

Produktnummer ABIN337082

Produktdetails anti-CTNNB1 Antikörper

Target: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte, Huhn, Xenopus laevis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CTNNB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Marke: IHC-plus™

Spezifität: Recognizes human b-catenin. Species cross-reactivity: chicken, mouse, rat and Xenopus.

Aufreinigung: Immunoaffinity purified

Immunogen: A 15 amino acid synthetic peptide derived from the C-terminus of the human and mouse b-catenin protein.

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.1 - 1 μg/mL), IHC, IHC-P (2.5 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. Western Blot after IP: 1-2 μg/mL. Cross-reactivity with a ~100kD protein of unknown identity is sometimes observed. Immunoprecipitation: 5-10 μg. Specifically immunoprecipitate the ~92kD b-Catenin protein from native cell lysates. Positive control: HeLa and A431 lysates.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Details zu CTNNB1

Target: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Andere Bezeichnung: CTNNB1 / Beta Catenin (CTNNB1 Produkte)

Synonyme: Jup antikoerper, LOC100217813 antikoerper, CTNNB1 antikoerper, CTNNB antikoerper, Bfc antikoerper, Catnb antikoerper, Mesc antikoerper, ctnnb antikoerper, id:ibd2058 antikoerper, wu:fb73e10 antikoerper, wu:fi81c06 antikoerper, wu:fk25h01 antikoerper, ctnnb1 antikoerper, CHBCAT antikoerper, MRD19 antikoerper, armadillo antikoerper, beta-catenin antikoerper, catenin beta 1 antikoerper, catenin (cadherin associated protein), beta 1 antikoerper, catenin (cadherin-associated protein), beta 1 antikoerper, catenin beta 1 S homeolog antikoerper, catenin beta 1 L homeolog antikoerper, CTNNB1 antikoerper, Ctnnb1 antikoerper, ctnnb1 antikoerper, ctnnb1.S antikoerper, ctnnb1.L antikoerper

Hintergrund: Name/Gene ID: CTNNB1
Family: Catenin

Synonyms: CTNNB1, Beta-catenin, Catenin beta-1, CTNNB, Beta Catenin

Gen-ID: 1499

UniProt: P35222

Pathways: WNT Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2