IL15RA Antikörper (Extracellular Domain)

Produktnummer ABIN327397

Produktdetails anti-IL15RA Antikörper

Target: IL15RA (Interleukin 15 Receptor, alpha (IL15RA))

Bindungsspezifität: Extracellular Domain

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser IL15RA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Neutralization (Neut), Functional Studies (Func)

Spezifität: Recognizes mouse IL-15Ra.

Aufreinigung: Immunoaffinity purified

Immunogen: NS0-derived rmIL-15Ra extracellular domain.

Type of Immunogen: Recombinant protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, Flo, Func, Neut, WB

Usage: Neutralization of bioactivity, Direct ELISA, Western Blot, Flow Cytometry. Neutralization of Mouse IL-15 R Bioactivity: The exact concentration of antibody required to neutralize the mouse cell surface IL-15 R mediated bioactivity is dependent on the cytokine concentration, cell type, growth conditions and the type of activity studied. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cell surface IL-15 R mediated IL-15 response on a responsive cell line, at a specific IL-15 concentration. As shown in Figures 1 and 2 on the next page, the ND50 for this lot of anti-mouse IL-15 R antibody was determined to be approximately 0.3-1.5 μg/mL in the presence of 0.8 ng/mL of human IL-15, using the CTLL-2 cell proliferation assay. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse IL-15 R. The detection limit for rmIL-15 R is approximately 0.1 ng/well. In this format, this antibody shows approximately 5 % cross-reactivity with rhIL-15 R and less than 1 % cross-reactivity with rhIL-2 R, rmIL-2 R and rmIL-2 R. Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse IL-15 R. The detection limit for rmIL-15 R is approximately 5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: This antibody can be used at 3-10 μg/mL/1 x10⁶ with an appropriate secondary antibody for indirect immunofluorescence staining of cells by flow cytometry.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 100 μg/mL.

Konzentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose. Reconstitution: Reconstitute, sterile PBS. If 1 mL of PBS is used, the antibody concentration will be 100 μg/mL.

Details zu IL15RA

Target: IL15RA (Interleukin 15 Receptor, alpha (IL15RA))

Andere Bezeichnung: IL15RA (IL15RA Produkte)

Hintergrund: Name/Gene ID: IL15RA
Family: Interleukin

Synonyms: IL15RA, CD215 antigen, IL-15RA, IL-15 receptor subunit alpha, IL-15R-alpha, Interleukin 15 receptor, alpha, CD215

Gen-ID: 3601

UniProt: Q13261

Pathways: JAK-STAT Signalweg