PODXL Antikörper (Extracellular Domain)

Produktnummer ABIN325654

Dieser Maus Monoklonal Antikörper erkennt spezifisch PODXL in WB, FACS und ICC. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für PODXL Antikörper (Extracellular Domain) (ABIN325654)

Target: PODXL (Podocalyxin-Like (PODXL))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PODXL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Produktdetails anti-PODXL Antikörper

Bindungsspezifität: Extracellular Domain

Spezifität: Recognizes human Podocalyxin. In Western Blots, this antibody does not cross-react with rmPodocalyxin or rhEndoglycan.

Aufreinigung: Protein G purified

Immunogen: NS0-derived rhPodocalyxin extracellular domain.

Type of Immunogen: Recombinant protein

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Approved: Flo, ICC (10 μg/mL), WB

Usage: Suitable for use in Flow Cytometry, Immunocytochemistry and Western Blot. Immunocytochemistry: 10 μg/mL using fixed cells. Flow Cytometry: Dilute this antibody to 0.1 mg/mL and add 5 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. Antibody binding may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Western Blot: t 1-2 μg/mL. Using a colorimetric detection system, the detection limit for rhPodocalyxin is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 200 μL sterile PBS

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.4, 5 % trehalose.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu PODXL

Target: PODXL (Podocalyxin-Like (PODXL))

Andere Bezeichnung: PODXL / Podocalyxin

Hintergrund: Name/Gene ID: PODXL

Synonyms: PODXL, GCTM-2 antigen, gp200, PCLP, PCLP-1, PCLP1, Podocalyxin-like protein 1, Podocalyxin, Podocalyxin-like, TRA-1-60, TRA-1-81

Gen-ID: 5420

UniProt: O00592

Pathways: Tube Formation