PD-1 Antikörper (Extracellular Domain)

Produktnummer ABIN325052

Der Ziege Polyklonal Anti-PD-1-Antikörper wurde für FACS, WB, IHC und ELISA validiert. Er ist geeignet, PD-1 in Proben von Maus zu detektieren.

Kurzübersicht für PD-1 Antikörper (Extracellular Domain) (ABIN325052)

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PD-1 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Produktdetails anti-PD-1 Antikörper

Bindungsspezifität: Extracellular Domain

Spezifität: Recognizes mouse PD-1 extracellular domain. Shows approximately 50 % cross-reactivity with rhPD-1.

Aufreinigung: Immunoaffinity purified

Immunogen: Sf21-derived rmPD-1 extracellular domain.

Type of Immunogen: Recombinant protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, Flo, IHC, WB

Usage: Western Blot, Immunohistochemistry, Flow Cytometry, Direct ELISA. Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse PD-1. The detection limit for rmPD-1 is approximately 5 ng/lane under non-reducing and reducing conditions. Immunohistochemistry: This antibody will detect PD-1 in cells and tissues. The working dilution is 2-15 μg/mL. Flow Cytometry: This antibody can be used at 3-10 μg/mL/1 x10⁶ with an appropriate secondary antibody for indirect immunofluorescence staining of cells by flow cytometry. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse PD-1. The detection limit for rmPD-1 is approximately 0.03 ng/well.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.5 mL sterile PBS

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.4, 5 % trehalose

Handhabung: Avoid frequent freeze thaws. Store undiluted.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu PD-1

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Andere Bezeichnung: PDCD1 / CD279 / PD-1

Hintergrund: Name/Gene ID: PDCD1
Family: Apoptosis

Synonyms: PDCD1, CD279, HPD-1, HPD-l, PD1, Protein PD-1, SLEB2, PD-1, CD279 antigen, Programmed cell death 1

Gen-ID: 5133

UniProt: Q15116

Pathways: Cancer Immune Checkpoints