XPA Antikörper (full length)

Produktnummer ABIN3200998

Dieses Maus Monoklonal-Antikörper erkennt spezifisch XPA in WB, ELISA und Inhibition. Er zeigt eine Reaktivität gegenüber Human und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für XPA Antikörper (full length) (ABIN3200998)

Target: XPA (Xeroderma Pigmentosum, Complementation Group A (XPA))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser XPA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Blocking Antibody (Inhibition)

Klon: 5F12

Produktdetails anti-XPA Antikörper

Bindungsspezifität: full length

Kreuzreaktivität: Maus

Kreuzreaktivität (Details): Expected to react also with mouse XPA from the sequence homology.

Aufreinigung: Purified

Sterilität: Sterile filtered

Immunogen: Recombinant full-length human XPA protein

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: 1. Western blotting: 0. 1~1 g/mL
2. ELISA
3. Inhibition of in vitro excision repair reaction
4. Inhibition of XPA interaction with ERCC1 and TFIIH
Other applications have not been tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.2, 50 % glycerol

Konservierungsmittel: Azide free

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: -20 C (For long term storage: -70 C)

Referenzen für anti-XPA Antikörper (ABIN3200998)

Saijo, Matsuda, Kuraoka, Tanaka: "Inhibition of nucleotide excision repair by anti-XPA monoclonal antibodies which interfere with binding to RPA, ERCC1, and TFIIH." in: Biochemical and biophysical research communications, Vol. 321, Issue 4, pp. 815-22, (2004) (PubMed).

Tanaka, Miura, Satokata, Miyamoto, Yoshida, Satoh, Kondo, Yasui, Okayama, Okada: "Analysis of a human DNA excision repair gene involved in group A xeroderma pigmentosum and containing a zinc-finger domain." in: Nature, Vol. 348, Issue 6296, pp. 73-6, (1990) (PubMed).

Details zu XPA

Target: XPA (Xeroderma Pigmentosum, Complementation Group A (XPA))

Andere Bezeichnung: XPA

Hintergrund: XP (Xeroderma pigmentosum) is an autosomal recessive human disease characterized by hypersensitivity to sunlight and a high incidence of skin cancer on sun-exposed skin. Cells from XP patients are hypersensitive to killing by UV irradiation because of a defect in nucleotide excision repair (NER). XP is classified into seven complementation groups (A~G) and a variant form. XPA shows the most severe symptoms. Products encoded by the XP genes function in repairing UV-induced cyclobutane pyrimidine dimmer and (6-4) photoproducts as well as chemically induced variety of DNA lesions. XPA protein consists of 273 amino acids and forms a complex with many proteins such as RPA, ERCC1, TFIIH,XAB1, and XAB2, which plays a role in early step of NER. The hybridoma 5F12 was constructed by Prof. K. Tanaka's group who first cloned the XPA gene.

UniProt: P23025

Pathways: DNA Reparatur