CASP5 Antikörper

Produktnummer ABIN319133

Der Kaninchen Polyklonal Anti-CASP5-Antikörper wurde für WB und IF validiert. Er ist geeignet, CASP5 in Proben von Human zu detektieren.

Kurzübersicht für CASP5 Antikörper (ABIN319133)

Target: CASP5 (Caspase 5, Apoptosis-Related Cysteine Peptidase (CASP5))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CASP5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Produktdetails anti-CASP5 Antikörper

Spezifität: This antibody recognises an epitope located within the internal region of human Caspase-5, a 47 kDa cysteine protease, otherwise known as ICH-3.

Aufreinigung: Antisera to human Caspase-5 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.

Immunogen: A 14 amino acid peptide from the centre of human Caspase-5.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western Blot: 1.0g/mL - 2.0g/mL, detects a band of approximately 48 kDa in Ramos celllysates. (Predicted precursor MWT 47.8 kDa)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline containing 0.02 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CASP5

Target: CASP5 (Caspase 5, Apoptosis-Related Cysteine Peptidase (CASP5))

Andere Bezeichnung: Caspase-5

Hintergrund: Caspase-5 has been shown to be expressed at higher levels in the colon, lung, spleen, small intestine and by peripheral blood lymphocytes. Caspase-5 is a member of a growing number of inflammatory caspases belonging to the Caspase-1 (ICE-type) subfamily, which require proteolytic processing of their inactive precursor into smaller active subunits. Unlike Caspase-1, Caspase-5 is not directly responsible for the activation of IL-1Beta from the pro-IL-1Beta precursor, but the presence of Caspase-5 does appear to enhance the processing of IL-1Beta. The in vivo expression of Caspase-5 is elevated by Lipopolysaccharide (LPS) and gene expression is also increased by Interferon gamma (IFNg). In vitro studies have shown cleavage of Caspase-5 by the serine protease Granzyme B, which plays a major role in Cytotoxic T lymphocyte (CTL)-induced apoptosis (1).Synonyms: CASP-5, CASP5, ICE(rel)-III, ICH-3 protease, ICH3, TY protease

Gen-ID: 838

NCBI Accession: NP_001129581

UniProt: P51878

Pathways: Inflammasome