ASK1 Antikörper (pSer83)

Produktnummer ABIN318809

Der Kaninchen Polyklonal Anti-ASK1-Antikörper wurde für WB und EIA validiert. Er ist geeignet, ASK1 in Proben von Human zu detektieren.

Kurzübersicht für ASK1 Antikörper (pSer83) (ABIN318809)

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ASK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-ASK1 Antikörper

Bindungsspezifität: AA 76-87, pSer83

Spezifität: This antibody detects Apoptosis Signal-regulating Kinase 1 (ASK1) phosphorylated at serine83. Akt in conjunction to Hsp90 phosphorylates ASK1 at Ser83 to inhibit ASK1 induced apoptosis.

Aufreinigung: Purified

Immunogen: KLH conjugated peptide corresponding to amino acids 76-87 of human ASK-1 protein.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1: 5,000 - 1: 10,000. Western Blot: 1: 1,000, detects a band of approximately 155 kDa in SW1353 cell lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 2.0mg/mL

Buffer: Phosphate buffered saline pH 7.2 containing 0.01 % Sodium Azide (NaN3)

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu ASK1

Target: ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))

Andere Bezeichnung: MEKK5 / ASK1

Hintergrund: ASK1 is a serine/threonine kinase also known as MAP kinase kinase kinase (MAPKKK), and plays an important role in the apoptotic response to various stimuli including oxidative stress, cytokines (e.g. TNF) and endoplasmic reticulum stress.Synonyms: Apoptosis signal-regulating kinase 1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, Mitogen-activated protein kinase kinase kinase 5

Gen-ID: 4217

NCBI Accession: NP_005914

UniProt: Q99683

Pathways: MAPK Signalweg, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response