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Complement C4A/C4B (AA 1241-1256) Antikörper

Dieses Anti--Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von in IHC (fro) und IF. Geeignet für Human.
Produktnummer ABIN318426

Kurzübersicht für Complement C4A/C4B (AA 1241-1256) Antikörper (ABIN318426)

Target

Complement C4A/C4B

Reaktivität

  • 9
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Wirt

  • 4
  • 4
  • 1
Kaninchen

Klonalität

  • 5
  • 4
Polyklonal

Konjugat

  • 9
Unkonjugiert

Applikation

  • 8
  • 5
  • 4
  • 3
  • 3
  • 1
  • 1
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
  • Bindungsspezifität

    • 2
    • 2
    • 2
    • 1
    AA 1241-1256

    Spezifität

    This antibody is specific for complement split product C4d.

    Aufreinigung

    Purified Ig prepared by affinity chromatography on Protein G

    Immunogen

    Synthetic peptide corresponding to amino acids 1241-1256 of C4 conjugated to Keyhole limpet Haemocyanin.

    Isotyp

    IgG
  • Applikationshinweise

    Immunohistochemistry on frozen sections: 1/20 - 1/80. Immunohistochemistry on paraffin sections: 1/20 - 1/80, Heat induced epitope retrieval isrecommended. Immunofluorescence: 1/20 - 1/80.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    0.2 mg/mL

    Buffer

    Phosphate buffered saline containing 0.09 % Sodium Azide (NaN3)

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C/-20 °C

    Informationen zur Lagerung

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target

    Complement C4A/C4B

    Hintergrund

    C4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway. Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.Prior to secretion, the single-chain precursor is enzymatically cleaved to yield the non-identical chains (alpha, beta and gamma). During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase.Synonyms: CPAMD2, CPAMD3, Complement Component 4

    Gen-ID

    720

    NCBI Accession

    NP_009224

    UniProt

    P0C0L4
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