Hexokinase 1 Antikörper (N-Term)

Produktnummer ABIN317095

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Hexokinase 1 in WB, IHC (p) und EIA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Hexokinase 1 Antikörper (N-Term) (ABIN317095)

Target: Hexokinase 1 (HK1)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Hexokinase 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Klon: 4D7

Produktdetails anti-Hexokinase 1 Antikörper

Bindungsspezifität: AA 1-917, N-Term

Spezifität: The antibody recognizes all four isoforms of Hexokinase (1~4) in recombinant protein.

Kreuzreaktivität (Details): Species reactivity (tested):Human, mouse (see pictures below).

Produktmerkmale: Synonyms: HK1, Hexokinase type I, Brain form hexokinase

Aufreinigung: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogen: Recombinant Human Hexokinase 1 (1-917 aa) purified from E. coli

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: ELISA. Western blot (1: 1,000). Immunofluorescence (1: 1,000). Immunohistochemistry on Paraffin Sections (5 μg/mL): This antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, containing 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu Hexokinase 1

Target: Hexokinase 1 (HK1)

Andere Bezeichnung: Hexokinase-1

Hintergrund: Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase1 is found in the adipose tissue and liver and encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. A deficiency in hexokinase 1 is implicated in the rare hereditary autosomal recessive disease known as nonspherocytic haemolytic anaemia.Synonyms: Brain form hexokinase, HK1, Hexokinase type I

Gen-ID: 3098

UniProt: P19367

Pathways: Carbohydrate Homeostasis, Warburg Effekt