ERN1 Antikörper (Internal Region)

Produktnummer ABIN308413

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch ERN1 in WB, ELISA und FACS. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für ERN1 Antikörper (Internal Region) (ABIN308413)

Target: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ERN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-ERN1 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: ERN1 / IRE1a

Sequenz: PNAHGKIKAM ISD

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Schwein, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-PNAHGKIKAMISD, from the internal region of the protein sequence according to NP_001424.3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 110 kDa band observed in Human Pancreas and Duodenum lysates (calculated MW of 110 kDa according to NP_001424.3). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:16000.

Kommentare:

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu ERN1

Target: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Andere Bezeichnung: ERN1

Hintergrund: ERN1, IRE1, endoplasmic reticulum to nucleus signaling 1, FLJ30999, IRE1P, MGC163277, MGC163279, ER to nucleus signalling 1, endoplasmic reticulum to nucleus signalling 1, inositol-requiring 1, protein kinase/endoribonuclease, IRE1a, IRE1-alpha

Gen-ID: 2081, 78943, 498013

NCBI Accession: NP_001424

Pathways: ER-Nucleus Signaling, Unfolded Protein Response