ATG7 Antikörper (C-Term)

Produktnummer ABIN307175

Der Kaninchen Polyklonal Anti-ATG7 Antikörper wurde für WB, IHC, ICC und IHC (p) validiert. Er ist geeignet, ATG7 in Proben von Human und Maus zu detektieren.

Kurzübersicht für ATG7 Antikörper (C-Term) (ABIN307175)

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATG7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-ATG7 Antikörper

Bindungsspezifität: C-Term

Spezifität: Recognizes human APG7.

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide, 17aa from near the carboxy terminus of human APG7 (Genbank accession No. NP_006386).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ICC, IHC, IHC-P (5 μg/mL), WB (0.5 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Positive control: Caco-2 cell lysate.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.2, 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Details zu ATG7

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Andere Bezeichnung: Apg7 / ATG7

Hintergrund: Name/Gene ID: ATG7

Synonyms: ATG7, Apg7, APG7L, APG7-LIKE, Autophagy related 7, Autophagy-related protein 7, HAGP7, GSA7

Gen-ID: 10533

NCBI Accession: NP_006386

UniProt: O95352

Pathways: Response to Water Deprivation, Autophagie