Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Kreuzreaktivität
Hund, Human, Maus, Ratte
Produktmerkmale
Calreticulin, a Ca2+ chaperone, is an abundant highly conserved calcium binding protein found in the lumen of the endoplasmic reticulum of eukaryotic cells. This lumen protein can be used to measure the intactness of membrane preparations. It is used as a marker for subcellular fractionation of cells.
Aufreinigung
Ammonium Sulfate Precipitation
Immunogen
Sheep were immunized with a recombinant protein derived from the carboxy terminus of the calreticulin protein fused to KLH.
Antibody can be used for Western blotting (1:1000-1:5000 dilution) and immunofluorescence (1:50 dilution). Optimal concentration should be evaluated by serial dilutions. Suitable for use against recombinant proteins conjugated to OVA, GST, His tags and other.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
Provided as solution in phosphate buffered saline with 0.08 % sodium azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C
Informationen zur Lagerung
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles