CDK4 Antikörper (C-Term)

Produktnummer ABIN306814

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch CDK4 in WB und IP. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für CDK4 Antikörper (C-Term) (ABIN306814)

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CDK4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Produktdetails anti-CDK4 Antikörper

Bindungsspezifität: C-Term

Kreuzreaktivität: Human

Produktmerkmale: p34cdk4 is a major catalytic subunit of mammalian D-type cyclins which is expressed by a gene found in human chromosome 12q13. The cdk4 protein is constitutively expressed throughout the cell cycle and it is the assembly and accumulation of functional cdk4/cyclin D complexes which is rate limiting for the progression through the G1 phase of the cell cycle. Once the complex is formed it must be activated by the phosphorylation of threonine 172 on the cdk4 subunit, a reaction mediated by a cdk-activating kinase (CAK). Complex formation and phosphorylation are independently regulated events. The phosphorylation of the cdk4 subunit is subject to regulation of both stimulatory and inhibitory cytokine signals, thereby allowing cdk4 to function as a mid G1 checkpoint. Independent of phosphorylation, cdk4 is also regulated by the binding of p16Ink4/mts1 while in complex with cyclin D.

Aufreinigung: Ammonium Sulfate Precipitation

Immunogen: TrpE-fusion protein containing the C-terminal 164 amino acids of human Cdk4

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Detects Cdk4 by Western blot in HeLa cell lysates. Can also be used for immunoprecipitation. Optimal concentration should be evaluated by serial dilutions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Details zu CDK4

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Andere Bezeichnung: cyclin dependent kinase 4

UniProt: P11802

Pathways: Zellzyklus, Mitotic G1-G1/S Phases, Regulation of Cell Size