Cyclin E1 Antikörper (full length)

Produktnummer ABIN306564

Der Maus Monoklonal anti-Cyclin E1 Antikörper (Klon 168) (ABIN306564) detektiert spezifisch Cyclin E1 in WB und IP. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Cyclin E1 Antikörper (full length) (ABIN306564)

Target: Cyclin E1 (CCNE1)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cyclin E1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Klon: 168

Produktdetails anti-Cyclin E1 Antikörper

Bindungsspezifität: full length

Verwendungszweck: Mouse anti Human cyclin E

Produktmerkmale: Human cyclin E was originally identified by complementation of a triple cln deletion in Saccharomyces cerevisiae. Following the identification of human cyclin E protein, researchers found that breast cancers and some other solid tumors exhibited both quantitative and qualitative alterations in cyclin E protein production. In particular, the alterations in cyclin E expression in breast cancer correlated with increasing stage and grade of the tumor. These results suggested that cyclin E might be useful as a prognostic marker. More recently, Geng et al generated a mouse strain in which the coding sequences for cyclin D1 was deleted and replaced with those of human cyclin E. Replacement of cyclin D1 with cyclin E rescued all phenotypic manifestations seen in the cyclin D1 deficient mice and restored normal development to cyclin D1-dependent tissues. Based on the results of these studies, it appears that cyclin E is the major downstream target of cyclin D1. In addition to functioning downstream of cyclin D1, recent studies with Xenopus egg extracts demonstrated that cyclin E localizes to the centrosome. In these studies CDK2-cyclin E activity was shown to be required for centrosome duplication during S phase. Taken together, these data suggest a mechanism that coordinates centrosome reproduction with cycles of DNA synthesis and mitosis.

Aufreinigung: Protein A/G Chromatography

Immunogen: Recombinant full-length human cyclin E protein

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Antibody detects human cyclin E protein by Western blot. Detects a 50 kDa band. Optimal concentration should be evaluated by serial dilutions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/ml

Buffer: phosphate buffered saline with 0.08 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Details zu Cyclin E1

Target: Cyclin E1 (CCNE1)

Andere Bezeichnung: cyclin E

Hintergrund: G1/S-specific cyclin E1, CCNE1

UniProt: P24864

Pathways: Zellzyklus, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases