Adenosine A2a Receptor Antikörper (3rd Intracellular Domain)

Produktnummer ABIN305729

Der Maus Monoklonal Anti-Adenosine A2a Receptor-Antikörper wurde für WB, IHC, FACS, IHC (p) und IHC (pfa) validiert. Er ist geeignet, Adenosine A2a Receptor in Proben von Human, Maus, Ratte, Hund, Kaninchen und Meerschweinchen zu detektieren.

Kurzübersicht für Adenosine A2a Receptor Antikörper (3rd Intracellular Domain) (ABIN305729)

Target: Adenosine A2a Receptor (ADORA2A)

Reaktivität: Human, Maus, Ratte, Hund, Kaninchen, Meerschweinchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Adenosine A2a Receptor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraformaldehyde) (IHC (pfa))

Klon: 7F6-G5-A2-7F6

Produktdetails anti-Adenosine A2a Receptor Antikörper

Bindungsspezifität: 3rd Intracellular Domain

Marke: IHC-plus™

Spezifität: Recognizes human adenosine receptor A2A at ~43kD.

Aufreinigung: Protein G purified

Immunogen: 6His-tagged full-length human recombinant A2A receptor. Epitope mapped to SQPLPGER in the third intracellular loop.

Type of Immunogen: Recombinant protein

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Approved: Flo, IHC, IHC-P (5 μg/mL), IHC-Pfa (1 μg/mL), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 1:1000-1:5000 detected Adenosine Receptor A2A in HEK293 cells expressing canine A1/A2A receptor chimeras.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.01M Tris-Glycine,  pH 7.4, 0.15M sodium chloride, 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Adenosine A2a Receptor

Target: Adenosine A2a Receptor (ADORA2A)

Andere Bezeichnung: ADORA2A/Adenosine A2A Receptor

Hintergrund: Name/Gene ID: ADORA2A
Subfamily: Adenosine
Family: GPCR

Synonyms: ADORA2A, Adenosine receptor A2a, ADORA2, A2a receptor, Adenosine A2a receptor, Adenosine A2 receptor, Adenosine receptor subtype A2a, HA2aR, RDC8

Gen-ID: 135

UniProt: P29274

Pathways: Neurotrophin Signalübertragung, cAMP Metabolic Process, Synaptic Membrane, Feeding Behaviour, Cancer Immune Checkpoints