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NONO Antikörper (N-Term)

NONO Reaktivität: Human, Maus, Ratte WB, IHC, IF, ICC, FACS Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN3044530
  • Target Alle NONO Antikörper anzeigen
    NONO (Non-POU Domain Containing, Octamer-Binding (NONO))
    Bindungsspezifität
    • 15
    • 8
    • 7
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-35, N-Term
    Reaktivität
    • 72
    • 36
    • 32
    • 15
    • 13
    • 6
    • 5
    • 5
    • 5
    • 3
    • 3
    • 1
    Human, Maus, Ratte
    Wirt
    • 65
    • 6
    • 2
    Kaninchen
    Klonalität
    • 58
    • 15
    Polyklonal
    Konjugat
    • 38
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser NONO Antikörper ist unkonjugiert
    Applikation
    • 55
    • 31
    • 23
    • 17
    • 13
    • 13
    • 12
    • 7
    • 6
    • 4
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
    Verwendungszweck
    Anti-nmt55/p54nrb/NONO Antibody Picoband®
    Sequenz
    MQSNKTFNLE KQNHTPRKHH QHHHQQQHHQ QQQQQ
    Kreuzreaktivität (Details)
    No cross-reactivity with other proteins.
    Produktmerkmale
    Anti-nmt55/p54nrb/NONO Antibody Picoband® (ABIN3044530). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
    Aufreinigung
    Immunogen affinity purified.
    Immunogen
    A synthetic peptide corresponding to a sequence at the N-terminus of human nmt55/p54nrb, identical to the related mouse and rat sequences.
    Isotyp
    IgG
    Top Product
    Discover our top product NONO Primärantikörper
  • Applikationshinweise
    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
    Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. "Entrez Gene: NONO Non-POU domain containing, octamer-binding". 2. Dong B, Horowitz DS, Kobayashi R, Krainer AR (Oct 1993). "Purification and cDNA cloning of HeLa cell p54nrb, a nuclear protein with two RNA recognition motifs and extensive homology to human splicing factor PSF and Drosophila NONA/BJ6". Nucleic Acids Res. 21 (17): 4085-92. 3. Traish AM, Huang YH, Ashba J, Pronovost M, Pavao M, McAneny DB, Moreland RB (Dec 1997). "Loss of expression of a 55 kDa nuclear protein (nmt55) in estrogen receptor-negative human breast cancer". Diagn. Mol. Pathol. 6(4): 209-21.
    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Konzentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target
    NONO (Non-POU Domain Containing, Octamer-Binding (NONO))
    Andere Bezeichnung
    NONO (NONO Produkte)
    Hintergrund

    Synonyms: Non-POU domain-containing octamer-binding protein,NonO protein,54 kDa nuclear RNA- and DNA-binding protein,55 kDa nuclear protein,DNA-binding p52/p100 complex, 52 kDa subunit,NMT55,p54 (nrb),p54nrb,NONO,NRB54,

    Tissue Specificity: Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Also found in a number of breast tumor cell lines. .

    Background: Non-POU domain-containing octamer-binding protein?is a?protein?that in humans is encoded by the?NONO gene. This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16.?

    Molekulargewicht
    60 kDa
    Gen-ID
    4841
    UniProt
    Q15233
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