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APEX1 Antikörper (Middle Region)

Der Kaninchen Polyklonal Anti-APEX1-Antikörper wurde für WB, IHC, IF und ICC validiert. Er ist geeignet, APEX1 in Proben von Human, Maus und Ratte zu detektieren.
Produktnummer ABIN3044417

Kurzübersicht für APEX1 Antikörper (Middle Region) (ABIN3044417)

Target

Alle APEX1 Antikörper anzeigen
APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Reaktivität

  • 96
  • 47
  • 43
  • 8
  • 7
  • 7
  • 7
  • 6
  • 6
  • 6
  • 5
  • 4
  • 2
  • 1
  • 1
  • 1
  • 1
Human, Maus, Ratte

Wirt

  • 80
  • 19
  • 2
Kaninchen

Klonalität

  • 80
  • 21
Polyklonal

Konjugat

  • 71
  • 4
  • 4
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser APEX1 Antikörper ist unkonjugiert

Applikation

  • 88
  • 33
  • 31
  • 27
  • 21
  • 15
  • 8
  • 8
  • 8
  • 3
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
  • Bindungsspezifität

    • 8
    • 8
    • 6
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 177-191, Middle Region

    Verwendungszweck

    Anti-APE1/APEX1 Antibody Picoband®

    Sequenz

    RGLVRLEYRQ RWDEA

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins

    Produktmerkmale

    Anti-APE1/APEX1 Antibody (ABIN3044417). Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    A synthetic peptide corresponding to a sequence in the middle region of human APE1, identical to the related rat and mouse sequences.

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
    Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human, Rat
    Immunofluorescence, 5 μg/mL, Human
    1. Akiyama, K., Seki, S., Oshida, T., Yoshida, M. C.Structure, promoter analysis and chromosomal assignment of the human APEX gene.Biochim. Biophys. Acta 1219: 15-25, 1994. 2. Chou, K.-M., Cheng, Y.-C.An exonucleolytic activity of human apurinic/apyrimidinic endonuclease on 3-prime mispaired DNA.Nature 415: 655-659, 2002. 3. Robson, C. N., Hochhauser, D., Craig, R., Rack, K., Buckle, V. J., Hickson, I. D.Structure of the human DNA repair gene HAP1 and its localisation to chromosome 14q11.2-12.Nucleic Acids Res. 20: 4417-4421, 1992.

    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

    Konservierungsmittel

    Thimerosal (Merthiolate), Sodium azide

    Vorsichtsmaßnahmen

    This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

    Haltbarkeit

    12 months
  • Target

    APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

    Andere Bezeichnung

    APEX1

    Hintergrund

    Synonyms: DNA- (apurinic or apyrimidinic site) lyase,3.1.-.-,4.2.99.18,APEX nuclease,APEN,Apurinic-apyrimidinic endonuclease 1,AP endonuclease 1,APE-1,REF-1,Redox factor-1,DNA- (apurinic or apyrimidinic site) lyase, mitochondrial,APEX1,APE, APE1, APEX, APX, HAP1, REF1,

    Tissue Specificity: Released primarily from activated T lymphocytes.

    Background: APEX1, also called apurinic endonuclease (APE), is a DNA repair enzyme having apurinic/apyrimidinic (AP) endonuclease, 3-prime,5-prime-exonuclease, DNA 3-prime repair diesterase, and DNA 3-prime-phosphatase activities. The human APEX1 gene consists of 5 exons spanning 2.64 kb and exists as a single copy in the haploid genome. Using in situ hybridization, the APEX1 gene is mapped to 14q11.2-q12. The predicted APEX1 protein, which contained probable nuclear transport signals, was identified as a member of a family of DNA repair enzymes found in lower organisms. The abundance of the large form of APEX1 was increased in leiomyoma extracts relative to myometrial tissue extracts, and the large form was dominant in cell lines derived from leiomyosarcomas. The exonuclease activity of nuclear APEX1 can remove the anti-HIV nucleoside analogs AZT and D4T from the 3-prime terminus of a nick more efficiently than can cytosolic exonucleases.

    Sequence Similarities: Belongs to the DNA repair enzymes AP/ExoA family.

    Molekulargewicht

    36 kDa

    UniProt

    P27695

    Pathways

    DNA Reparatur, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus
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