CTNNB1 Antikörper (C-Term)

Produktnummer ABIN3044344

Der Kaninchen Polyklonal Anti-CTNNB1-Antikörper wurde für WB, IHC, IF, ICC und IHC (fro) validiert. Er ist geeignet, CTNNB1 in Proben von Human, Maus und Ratte zu detektieren. Es sind 15+ Publikationen verfügbar.

Kurzübersicht für CTNNB1 Antikörper (C-Term) (ABIN3044344)

Target: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CTNNB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-CTNNB1 Antikörper

Bindungsspezifität: AA 764-781, C-Term

Verwendungszweck: Anti-beta Catenin/CTNNB1 Antibody Picoband®

Sequenz: DGLPPGDSNQ LAWFDTDL

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-beta Catenin/CTNNB1 Antibody (ABIN3044344). Tested in IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human beta Catenin, identical to the related rat and mouse sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Mouse, Rat, Human
Immunocytochemistry , 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
1. N. Peyrieras, D. Louvard and F. Jacob. "Characterization of antigens recognized by monoclonal and polyclonal antibodies ed against uvomorulin" in Proceedings of the National Academy of Sciences of the United States of America (1985) Volume 82, pages 8067-8071. 2. Thompson MD, Monga SP (2007). "WNT/beta-catenin signaling in liver health and disease". Hepatology 45 (5): 1298-305.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-CTNNB1 Antikörper (ABIN3044344)

Ma, Lv, Sun, Ma, Xing, Wang, Sun, Wang, Li, Li, Zhao: "Naringin ameliorates bone loss induced by sciatic neurectomy and increases Semaphorin 3A expression in denervated bone." in: Scientific reports, Vol. 6, pp. 24562, (2018) (PubMed).

Jia, Ma, Lv, Ma, Xu, Yang, Tian, Wang, Sun, Xu, Fu, Zhao: "Oestrogen and parathyroid hormone alleviate lumbar intervertebral disc degeneration in ovariectomized rats and enhance Wnt/β-catenin pathway activity." in: Scientific reports, Vol. 6, pp. 27521, (2018) (PubMed).

Cong, Fu, Zhang, Zhao, Zhang: "Effects of atorvastatin on porcine aqueous humour outflow and trabecular meshwork cells." in: Experimental and therapeutic medicine, Vol. 15, Issue 1, pp. 210-216, (2018) (PubMed).

Guo, Yi, Li, Fu, Li: "Snail1 is positively correlated with atrial fibrosis in patients with atrial fibrillation and rheumatic heart disease." in: Experimental and therapeutic medicine, Vol. 14, Issue 5, pp. 4231-4237, (2017) (PubMed).

Pan, An, Meng, Li, Ren, Guan: "MgCl2 and ZnCl2 promote human umbilical vein endothelial cell migration and invasion and stimulate epithelial-mesenchymal transition via the Wnt/β-catenin pathway." in: Experimental and therapeutic medicine, Vol. 14, Issue 5, pp. 4663-4670, (2017) (PubMed).

Fu, Jiao, Zheng, Liang, Hu: "Combination of lithium chloride and pEGFP-N1-BmK CT effectively decreases proliferation and migration of C6 glioma cells." in: Cytotechnology, Vol. 68, Issue 2, pp. 197-202, (2016) (PubMed).

Lei, Lai, Bai, Qiu, Yang, Liao, Chuong, Yang, Lian, Zhong: "Prolonged overexpression of Wnt10b induces epidermal keratinocyte transformation through activating EGF pathway." in: Histochemistry and cell biology, Vol. 144, Issue 3, pp. 209-21, (2016) (PubMed).

Chen, Xue, Shen, Mu, Fu: "Curcumin alleviates glucocorticoid-induced osteoporosis through the regulation of the Wnt signaling pathway." in: International journal of molecular medicine, Vol. 37, Issue 2, pp. 329-38, (2016) (PubMed).

Shan, Lv, Zhao, Liu, Sun, Xi, Xiao, Li: "Wnt/β-catenin pathway is required for epithelial to mesenchymal transition in CXCL12 over expressed breast cancer cells." in: International journal of clinical and experimental pathology, Vol. 8, Issue 10, pp. 12357-67, (2016) (PubMed).

Lin, Sun, Wang, Zheng, Zhang, Zheng: "Celastrol Ameliorates Ulcerative Colitis-Related Colorectal Cancer in Mice via Suppressing Inflammatory Responses and Epithelial-Mesenchymal Transition." in: Frontiers in pharmacology, Vol. 6, pp. 320, (2016) (PubMed).

Wang, Ge, Zhang, Chen, Hu, Li, Ye: "Targeted p53 activation by saRNA suppresses human bladder cancer cells growth and metastasis." in: Journal of experimental & clinical cancer research : CR, Vol. 35, pp. 53, (2016) (PubMed).

Hao, Wen, Li, Wang, Ni, Wang, Wang, Sun, Fan, Mao: "LiCl inhibits PRRSV infection by enhancing Wnt/?-catenin pathway and suppressing inflammatory responses." in: Antiviral research, Vol. 117, pp. 99-109, (2015) (PubMed).

Zhao, Li, Gao, Wang, Yan, Zhan, Liu, Mao, Chen, Wang et al.: "Design, synthesis and biological evaluation of N-alkyl or aryl substituted isoindigo derivatives as potential dual cyclin-dependent kinase 2 (CDK2)/glycogen synthase kinase 3? (GSK-3?) ..." in: European journal of medicinal chemistry, Vol. 86, pp. 165-74, (2014) (PubMed).

Lyu, Wang, Wang, Ding, Liu, Yuan, Shi, Wang, Wang, Chen: "Maintaining the pluripotency of mouse embryonic stem cells on gold nanoparticle layers with nanoscale but not microscale surface roughness." in: Nanoscale, Vol. 6, Issue 12, pp. 6959-69, (2014) (PubMed).

Lei, Guo, Qiu, Lai, Yang, Widelitz, Chuong, Lian, Yang: "Modulating hair follicle size with Wnt10b/DKK1 during hair regeneration." in: Experimental dermatology, Vol. 23, Issue 6, pp. 407-13, (2014) (PubMed).

Details zu CTNNB1

Target: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Andere Bezeichnung: CTNNB1

Hintergrund:

Synonyms: Catenin beta-1,Beta-catenin,CTNNB1,CTNNB,OK/SW-cl.35, PRO2286,

Tissue Specificity: Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. Present in cortical neurons (at protein level). .

Background: Catenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. The first two catenins that were identified became known as alpha-catenin and beta-catenin. Alpha-catenin can bind to beta-catenin and can also bind actin. Beta-catenin binds the cytoplasmic domain of some cadherins. Beta-catenin is an adherens junction protein. It plays an important role in various aspects of liver biology including liver development (both embryonic and postnatal), liver regeneration following partial hepatectomy. HGF-induced hepatpomegaly, liver zonation, and pathogenesis of liver cancer.

Sequence Similarities: Belongs to the beta-catenin family.

Molekulargewicht: 95 kDa

UniProt: P35222

Pathways: WNT Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2