Deoxyuridine Triphosphatase (DUT) (AA 212-229), (C-Term) Antikörper

Produktnummer ABIN3044322

Dieses Anti--Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von in WB und IHC. Geeignet für Human, Ratte und Maus.

Kurzübersicht für Deoxyuridine Triphosphatase (DUT) (AA 212-229), (C-Term) Antikörper (ABIN3044322)

Target: Deoxyuridine Triphosphatase (DUT)

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails

Bindungsspezifität: AA 212-229, C-Term

Verwendungszweck: Anti-DUT Antibody Picoband®

Sequenz: KKGDRIAQLI CERIFYPE

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-DUT Antibody (ABIN3044322). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human DUT, different from the related rat and mouse sequences by two amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, Mouse
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Studebaker AW, Lafuse WP, Kloesel R, Williams MV.Modulation of human dUTPase using small interfering RNA. Biochem Biophys Res Commun. 2005 Feb 4, 327(1):306-10. 2. McIntosh EM, Ager DD, Gadsden MH, Haynes RH.Human dUTP pyrophosphatase: cDNA sequence and potential biological importance of the enzyme. Proc Natl Acad Sci U S A. 1992 Sep 1, 89(17):8020-4. 3. Chu R, Lin Y, Rao MS, Reddy JK.Cloning and identification of rat deoxyuridine triphosphatase as an inhibitor of peroxisome proliferator-activated receptor alpha. J Biol Chem. 1996 Nov 1, 271(44):27670-6. 4. Ladner RD, Caradonna SJ.The human dUTPase gene encodes both nuclear and mitochondrial isoforms.Differential expression of the isoforms and characterization of a cDNA encoding the mitochondrial species. J Biol Chem. 1997 Jul 25, 272(30):19072-80. 5. Ladner RD.The role of dUTPase and uracil-DNA repair in cancer chemotherapy. Curr Protein Pept Sci. 2001 Dec, 2(4):361-70.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: Deoxyuridine Triphosphatase (DUT)

Andere Bezeichnung: DUT

Hintergrund:

Synonyms: Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial,dUTPase,3.6.1.23,dUTP pyrophosphatase,DUT,

Tissue Specificity: Found in a variety of tissues. Isoform 3 expression is constitutive, while isoform 2 expression correlates with the onset of DNA replication (at protein level). Isoform 2 degradation coincides with the cessation of nuclear DNA replication (at protein level). .

Background: Deoxyuridine triphosphate nucleotidohydrolase (dUTPase) is responsible for maintaining low intracellular levels of dUTP, thus preventing the incorporation of dUTP into DNA. dUTPase activity/expression can be down-regulated using siRNA specifically targeted to dUTPase mRNA and dUTPase plays a role in DNA nucleotide metabolism. This protein, present predominantly in the cytoplasm, contains 252 amino acids with a Mr of 26,704. It exhibits 35 % identity with the E.coli dUTPase and 53 % identity with the Saccharomyces cerevisiae enzyme. The nuclear and mitochondrial forms of dUTPase are encoded by the same gene with isoform-specific transcripts arising through the use of alternative 5-prime exons. Human dUTPase exhibits 92 % identity with rat. Moreover, this enzyme has profound effects on the efficacy of agents that target thymidylate biosynthesis.

Sequence Similarities: Belongs to the dUTPase family.

Molekulargewicht: 27 kDa

UniProt: P33316